Document Detail


Critical role of the C-terminus in the biological activities of human tumour necrosis factor-alpha.
MedLine Citation:
PMID:  2269475     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alterations of the C-terminal amino acid sequence of human tumour necrosis factor-alpha (hTNF-alpha) caused significant changes in its biological activity. Thus shortening of the C-terminus by removal of two or three amino acids led to a very marked loss of cytotoxic activity. Other, more subtle changes introduced by site-directed mutagenesis resulted in a less drastic reduction in cytotoxicity. The mitogenic activity towards human fibroblasts of the hTNF-alpha was reduced in parallel with the loss of cytotoxicity. These results suggest that the C-terminal amino acids of hTNF-alpha are critical for its biological actions and that they may be part of the receptor-binding site.
Authors:
K Gase; V G Korobko; H G Wisniewski; J Le; V N Dobrynin; S A Filippov; W Gutsche; Y N Maksimova; B Schlott; L N Shingarova
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Immunology     Volume:  71     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-02-19     Completed Date:  1991-02-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  368-71     Citation Subset:  IM    
Affiliation:
Zentralinstitut für Mikrobiologie und Experimentelle Therapie, Akademie der Wissenschaften der DDR, Jena.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Cytotoxicity, Immunologic
Humans
Mitosis / immunology
Molecular Sequence Data
Mutagenesis, Site-Directed / immunology
Recombinant Proteins / chemistry,  immunology
Tumor Necrosis Factor-alpha / chemistry*,  immunology
Grant Support
ID/Acronym/Agency:
AI28993/AI/NIAID NIH HHS; CA47304/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha
Comments/Corrections

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