Document Detail


Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment.
MedLine Citation:
PMID:  17334369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lymphocyte homing is mediated by specific interaction between L-selectin on lymphocytes and the carbohydrate ligand 6-sulfo sialyl Lewis X on high endothelial venules. Here we generated mice lacking both core 1 extension and core 2 branching enzymes to assess the functions of O-glycan-borne L-selectin ligands in vivo. Mutant mice maintained robust lymphocyte homing, yet they lacked O-glycan L-selectin ligands. Biochemical analyses identified a class of N-glycans bearing the 6-sulfo sialyl Lewis X L-selectin ligand in high endothelial venules. These N-glycans supported the binding of L-selectin to high endothelial venules in vitro and contributed in vivo to O-glycan-independent lymphocyte homing in wild-type and mutant mice. Our results demonstrate the critical function of N-glycan-linked 6-sulfo sialyl Lewis X in L-selectin-dependent lymphocyte homing and recruitment.
Authors:
Junya Mitoma; Xingfeng Bao; Bronislawa Petryanik; Patrick Schaerli; Jean-Marc Gauguet; Shin-Yi Yu; Hiroto Kawashima; Hideo Saito; Kazuaki Ohtsubo; Jamey D Marth; Kay-Hooi Khoo; Ulrich H von Andrian; John B Lowe; Minoru Fukuda
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-03-04
Journal Detail:
Title:  Nature immunology     Volume:  8     ISSN:  1529-2908     ISO Abbreviation:  Nat. Immunol.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-21     Completed Date:  2007-05-17     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  100941354     Medline TA:  Nat Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  409-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Surface / immunology
Cell Adhesion / immunology
Dermatitis, Contact / immunology
Endothelium, Lymphatic / cytology,  immunology
L-Selectin / immunology*
Lymph Nodes / cytology,  immunology*
Lymphocytes / cytology,  immunology*
Membrane Proteins / immunology
Mice
Mice, Knockout
Microscopy, Fluorescence
Oligosaccharides / immunology*
Polysaccharides / immunology*
Grant Support
ID/Acronym/Agency:
AI061663/AI/NIAID NIH HHS; AI069259/AI/NIAID NIH HHS; AR42689/AR/NIAMS NIH HHS; CA48737/CA/NCI NIH HHS; DK48247/DK/NIDDK NIH HHS; P01 CA71932/CA/NCI NIH HHS; R01 AI069259/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/L-selectin counter-receptors; 0/Membrane Proteins; 0/Oligosaccharides; 0/Polysaccharides; 0/sialyl-2-3-(6'-sulfo)galactosyl-1-4-(fucopyranosyl-1-3)-N-acetylglucosamine; 126880-86-2/L-Selectin
Comments/Corrections
Comment In:
Nat Immunol. 2007 Apr;8(4):339-41   [PMID:  17375095 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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