Document Detail


Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin.
MedLine Citation:
PMID:  18724372     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Inflammasomes are cytosolic multiprotein complexes that sense microbial infection and trigger cytokine production and cell death. However, the molecular components of inflammasomes and what they sense remain poorly defined. Here we demonstrate that 35 amino acids of the carboxyl terminus of flagellin triggered inflammasome activation in the absence of bacterial contaminants or secretion systems. To further elucidate the host flagellin-sensing pathway, we generated mice deficient in the intracellular sensor Naip5. These mice failed to activate the inflammasome in response to the 35 amino acids of flagellin or in response to Legionella pneumophila infection. Our data clarify the molecular basis for the cytosolic response to flagellin.
Authors:
Karla L Lightfield; Jenny Persson; Sky W Brubaker; Chelsea E Witte; Jakob von Moltke; Eric A Dunipace; Thomas Henry; Yao-Hui Sun; Dragana Cado; William F Dietrich; Denise M Monack; Renée M Tsolis; Russell E Vance
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-08-24
Journal Detail:
Title:  Nature immunology     Volume:  9     ISSN:  1529-2916     ISO Abbreviation:  Nat. Immunol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-18     Completed Date:  2008-10-14     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  100941354     Medline TA:  Nat Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1171-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Motifs / immunology
Animals
Apoptosis Regulatory Proteins / immunology,  metabolism
Calcium-Binding Proteins / immunology,  metabolism
Cytosol
Enzyme-Linked Immunosorbent Assay
Flagellin / chemistry,  immunology*
Immunoblotting
Legionella pneumophila / immunology
Legionnaires' Disease / immunology
Macrophages / immunology*,  microbiology
Mice
Multiprotein Complexes / immunology*
Neuronal Apoptosis-Inhibitory Protein / genetics,  immunology*
Toll-Like Receptor 5 / immunology,  metabolism
Transduction, Genetic
Grant Support
ID/Acronym/Agency:
AI070739/AI/NIAID NIH HHS; AI075039/AI/NIAID NIH HHS; K22 AI070739/AI/NIAID NIH HHS; K22 AI070739-02/AI/NIAID NIH HHS; R01 AI075039/AI/NIAID NIH HHS; R01 AI075039-01A2/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Birc1e protein, mouse; 0/Calcium-Binding Proteins; 0/Ipaf protein, mouse; 0/Multiprotein Complexes; 0/Neuronal Apoptosis-Inhibitory Protein; 0/Toll-Like Receptor 5; 12777-81-0/Flagellin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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