Document Detail

Critical evaluation of postmortem changes in human autopsy cisternal fluid. Enzymes, electrolytes, acid-base balance, glucose and glycolysis, free amino acids and ammonia. Correlation to total brain ischemia.
MedLine Citation:
PMID:  8515212     Owner:  NLM     Status:  MEDLINE    
By studying early postmortem changes in cerebrospinal fluid (CSF) it is possible to draw conclusions as to premortem focal brain cell injury and terminal brain ischemia. Cisternal fluid (CF) from 40 different adult cadavers with no known neurological disorder was analyzed and compared with known in vivo values. They were divided into four groups (n = 10 in each group), CF samples taken 2, 4, 10, and 24 h after death. The enzyme activity of CK and CK-BB (EC increased linearly and statistically significantly 4-24 h postmortem (P < 0.001) the 2 h values being already 10 to 20 times higher than in vivo, LD and its isoenzymes 1 to 3 (EC distinctly 10 to 24 h after death. Glucose and pyruvate concentrations in the CF declined, as did Na+ and Cl-. Lactate and K+ increased over time. The earliest statistically significant changes between different timepoints were seen in lactate, pyruvate and K+ concentrations. The GABA concentration was already more than 170 times at 2 h postmortem, and glutamate more than 20 times higher than in vivo. The concentrations of alanine, glycine, lysine, histidine, isoleucine, phenylalanine, and tyrosine were 2 to 3 times higher at 2 h postmortem than during life. The concentrations of all amino acids and ammonia increased linearly and statistically significantly (P < 0.001) in the CF 4 to 24 h postmortem.
J T Kärkelä
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of forensic sciences     Volume:  38     ISSN:  0022-1198     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-07-19     Completed Date:  1993-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  603-16     Citation Subset:  IM    
Department of Anaesthesia, Tampere University Hospital, Finland.
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MeSH Terms
Acid-Base Equilibrium
Amino Acids / cerebrospinal fluid
Ammonia / cerebrospinal fluid
Body Fluids / chemistry*,  metabolism
Brain Death / cerebrospinal fluid*,  pathology*,  physiopathology
Carbon Dioxide / blood,  cerebrospinal fluid
Creatine Kinase / cerebrospinal fluid,  metabolism
Electrolytes / cerebrospinal fluid
Glucose / cerebrospinal fluid
Glycolysis / physiology
Postmortem Changes*
Pyruvates / cerebrospinal fluid
Pyruvic Acid
Reg. No./Substance:
0/Amino Acids; 0/Electrolytes; 0/Pyruvates; 124-38-9/Carbon Dioxide; 127-17-3/Pyruvic Acid; 50-99-7/Glucose; 7664-41-7/Ammonia; EC Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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