| Critical role of Pcid2 in B cell survival through the regulation of MAD2 expression. | |
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MedLine Citation:
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PMID: 20870947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mitotic checkpoint is essential for maintaining genomic stability in differentiating B cells undergoing genetic alterations of the Ig gene. In this study, using real-time RT-PCR and in situ RNA hybridization, we demonstrated that MAD2 mRNA export is selectively regulated by Pcid2/Thp1. Pcid2 small interfering RNA induced a cell-cycle abnormality with increased apoptosis and polyploidy, as previously observed in MAD2-knockdown cells. Pcid2 small interfering RNA reduced MAD2 expression, but not the expression of other cell-cycle checkpoint proteins, such as MAD1 and BUBR1, or the cell-cycle-associated proteins, cyclin A, cyclin B1, and cyclin-dependent kinase 1. In mouse B lineage cells, Pcid2 transcripts appeared in a stage-dependent manner at high levels in bone marrow pre-B and immature B cells, and in spleen transitional 1 and follicular B cells, but at lower levels in pro-B, transitional 2, and marginal zone B cells, suggesting a stage-dependent requirement for MAD2 regulation. Cd19-cre-derived targeting of the Pcid2 gene induced a mature B cell deficiency in mice. These findings indicate that Pcid2 is essential for B cell survival through the regulation of MAD2 expression during B cell differentiation. |
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Authors:
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Teruo Nakaya; Kazuhiko Kuwahara; Kazutaka Ohta; Masahiro Kitabatake; Teppei Toda; Naoki Takeda; Tokio Tani; Eisaku Kondo; Nobuo Sakaguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-24 |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 185 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-21 Completed Date: 2010-11-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 5180-7 Citation Subset: AIM; IM |
Affiliation:
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Department of Immunology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / cytology*, immunology, metabolism Cell Cycle / genetics, immunology Cell Cycle Proteins / biosynthesis, genetics, immunology, metabolism* Cell Differentiation / immunology Cell Survival / genetics, immunology Gene Expression Gene Expression Regulation / immunology* Immunoblotting Immunohistochemistry In Situ Hybridization Mice Mice, Knockout RNA, Small Interfering Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/MAD2 protein, mouse; 0/RNA, Small Interfering |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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