Document Detail


A critical role for LTA4H in limiting chronic pulmonary neutrophilic inflammation.
MedLine Citation:
PMID:  20813919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leukotriene A(4) hydrolase (LTA(4)H) is a proinflammatory enzyme that generates the inflammatory mediator leukotriene B(4) (LTB(4)). LTA(4)H also possesses aminopeptidase activity with unknown substrate and physiological importance; we identified the neutrophil chemoattractant proline-glycine-proline (PGP) as this physiological substrate. PGP is a biomarker for chronic obstructive pulmonary disease (COPD) and is implicated in neutrophil persistence in the lung. In acute neutrophil-driven inflammation, PGP was degraded by LTA(4)H, which facilitated the resolution of inflammation. In contrast, cigarette smoke, a major risk factor for the development of COPD, selectively inhibited LTA(4)H aminopeptidase activity, which led to the accumulation of PGP and neutrophils. These studies imply that therapeutic strategies inhibiting LTA(4)H to prevent LTB(4) generation may not reduce neutrophil recruitment because of elevated levels of PGP.
Authors:
Robert J Snelgrove; Patricia L Jackson; Matthew T Hardison; Brett D Noerager; Andrew Kinloch; Amit Gaggar; Suresh Shastry; Steven M Rowe; Yun M Shim; Tracy Hussell; J Edwin Blalock
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-02
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  330     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-08     Completed Date:  2010-10-20     Revised Date:  2011-07-28    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  90-4     Citation Subset:  IM    
Affiliation:
Division of Pulmonary, Allergy and Critical Care Medicine, University of Alabama at Birmingham Lung Health Center, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA. rjs198@imperial.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Animals
Bronchoalveolar Lavage Fluid / chemistry
Cells, Cultured
Chemokines, CXC / metabolism
Chemotaxis, Leukocyte
Epoxide Hydrolases / antagonists & inhibitors,  isolation & purification,  metabolism*
Female
Humans
Inflammation
Leukotriene B4 / metabolism
Lung / immunology*,  metabolism,  pathology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Neutrophils / enzymology,  immunology,  physiology*
Oligopeptides / metabolism*
Orthomyxoviridae Infections / immunology,  metabolism,  pathology
Pneumococcal Infections / immunology,  metabolism,  pathology
Pneumonia / immunology*,  metabolism,  pathology,  therapy
Proline / analogs & derivatives*,  metabolism
Pulmonary Disease, Chronic Obstructive / immunology,  metabolism,  pathology
Smoke*
Tobacco
Grant Support
ID/Acronym/Agency:
082727/Z/07/Z//Wellcome Trust; 1K23DK075788/DK/NIDDK NIH HHS; 1R03DK084110-01/DK/NIDDK NIH HHS; HL07783/HL/NHLBI NIH HHS; HL087824/HL/NHLBI NIH HHS; HL090999/HL/NHLBI NIH HHS; HL102371-A1/HL/NHLBI NIH HHS; K08HL091127/HL/NHLBI NIH HHS; P171/03/C1/048//Medical Research Council; P30 DK079337/DK/NIDDK NIH HHS; P30AR050948/AR/NIAMS NIH HHS; P30CA13148/CA/NCI NIH HHS; P50 AT00477/AT/NCCAM NIH HHS; R01 HL077783-05/HL/NHLBI NIH HHS; R01 HL077783-06/HL/NHLBI NIH HHS; R01 HL087824-02/HL/NHLBI NIH HHS; R01 HL090999-02S1/HL/NHLBI NIH HHS; R01 HL090999-04/HL/NHLBI NIH HHS; R01 HL102371-02/HL/NHLBI NIH HHS; RR19231/RR/NCRR NIH HHS; U54CA100949/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Chemokines, CXC; 0/Oligopeptides; 0/Smoke; 0/prolyl-glycyl-proline; 147-85-3/Proline; 71160-24-2/Leukotriene B4; EC 3.3.2.-/Epoxide Hydrolases; EC 3.3.2.-/leukotriene A4 hydrolase
Comments/Corrections
Comment In:
Science. 2010 Oct 1;330(6000):40-1   [PMID:  20929796 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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