Document Detail


Cripto-1 enhances migration and branching morphogenesis of mouse mammary epithelial cells.
MedLine Citation:
PMID:  11339828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cripto-1 is an EGF-CFC protein that performs an important role during early vertebrate development and is overexpressed in several types of human cancer. In the present study mouse EpH4, NMuMG, and TAC-2 mammary epithelial cells that are negative for endogenous cripto-1 expression were transfected with the murine cripto-1 cDNA. Cripto-1-transfected cell lines exhibited functional and physiological differences from the original cell lines including enhanced anchorage-independent growth in soft agar (EpH4 cells), growth in serum-free medium, increased proliferation, and formation of branching, duct-like structures when grown in a three-dimensional collagen type I matrix. Furthermore, cripto-1-expressing cell lines showed elevated migration in vitro in Boyden chamber and wound-healing assays. These results indicate that cripto-1 can function through an autocrine pathway that enables mammary epithelial cells to undergo an epithelial to mesenchymal transition.
Authors:
C Wechselberger; A D Ebert; C Bianco; N I Khan; Y Sun; B Wallace-Jones; R Montesano; D S Salomon
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental cell research     Volume:  266     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-07     Completed Date:  2001-06-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  95-105     Citation Subset:  IM    
Affiliation:
Laboratory of Tumor Immunology and Biology, Tumor Growth Factor Section, National Cancer Institute, National Institutes of Health, Building 10, Room 5B39, 10 Center Drive, Bethesda, Maryland 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / drug effects,  metabolism
Animals
Biological Assay / methods
Breast / drug effects*,  growth & development*,  metabolism
Calcium-Calmodulin-Dependent Protein Kinases / metabolism
Cell Differentiation / drug effects*,  physiology
Cell Division / drug effects,  physiology
Cell Movement / drug effects*,  physiology
Cell Size / drug effects*,  physiology
Cells, Cultured / drug effects,  metabolism
Culture Media, Serum-Free / pharmacology
Epidermal Growth Factor*
Epithelial Cells / cytology,  drug effects*,  metabolism
Female
Gene Expression Regulation, Neoplastic / drug effects,  physiology
Glycogen Synthase Kinase 3
Membrane Glycoproteins*
Mice
Mice, Inbred BALB C
Mitogen-Activated Protein Kinases / drug effects,  metabolism
Neoplasm Metastasis / physiopathology
Neoplasm Proteins / genetics,  metabolism*,  pharmacology*
RNA, Messenger / metabolism
Transgenes / drug effects,  physiology
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Membrane Glycoproteins; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/Tdgf1 protein, mouse; 62229-50-9/Epidermal Growth Factor; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.26/Glycogen Synthase Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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