| Crevicular fluid glutathione levels in periodontitis and the effect of non-surgical therapy. | |
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MedLine Citation:
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PMID: 19968740 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To quantify reduced and oxidized glutathione (GSH and GSSG) levels in gingival crevicular fluid (GCF) of periodontitis patients pre-therapy (versus periodontally healthy controls) and ascertain whether successful non-surgical therapy alters glutathione levels. MATERIALS AND METHODS: Thirty-second GCF samples (6/subject) were collected on Periopaper() strips from starved, non-smokers (n=20; mean age 43.6 years) with chronic periodontitis, before and 3 months after non-surgical therapy, and periodontally healthy, age- and gender-matched controls (n=20). GSH and GSSG levels were determined using reversed-phase high-performance liquid chromatography with fluorescence detection. RESULTS: Lower concentrations of GSH (p<0.01) and GSSG (p<0.05) were detected in GCF from patients (pre- and post-therapy) than controls and treatment had no significant effect. Amounts per 30-second sample did not differ between patients and controls. However, the amount of GSSG per 30-second sample decreased in patients after therapy (p<0.05). Consequently, therapy increased the GSH:GSSG ratio (p<0.05) in patients compared with the controls (p=0.8). CONCLUSION: These data demonstrate high concentrations of GSH within GCF, which are compromised in chronic periodontitis. While therapy does not appear to fully restore GSH concentrations in GCF, it does restore the redox balance (GSH:GSSG ratio), suggesting that the abnormal redox balance arises secondary to oxidative stress resulting from periodontal inflammation. |
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Authors:
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Melissa M Grant; Gareth R Brock; John B Matthews; Iain L C Chapple |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-24 |
Journal Detail:
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Title: Journal of clinical periodontology Volume: 37 ISSN: 1600-051X ISO Abbreviation: J. Clin. Periodontol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-25 Completed Date: 2010-04-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0425123 Medline TA: J Clin Periodontol Country: Denmark |
Other Details:
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Languages: eng Pagination: 17-23 Citation Subset: D; IM |
Affiliation:
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Periodontology Research Group, School of Dentistry, University of Birmingham, Birmingham, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Alveolar Bone Loss / metabolism, therapy Case-Control Studies Chronic Periodontitis / metabolism*, therapy Dental Scaling Female Follow-Up Studies Gingival Crevicular Fluid / chemistry* Gingival Hemorrhage / metabolism, therapy Glutathione / analysis* Glutathione Disulfide / analysis Humans Male Oxidation-Reduction Periodontal Attachment Loss / metabolism, therapy Periodontal Pocket / metabolism, therapy Root Planing Subgingival Curettage |
| Chemical | |
Reg. No./Substance:
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27025-41-8/Glutathione Disulfide; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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