Document Detail


Creation of bony microenvironment with CaP and cell-derived ECM to enhance human bone-marrow MSC behavior and delivery of BMP-2.
MedLine Citation:
PMID:  21632105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extracellular matrix (ECM) comprises a rich meshwork of proteins and proteoglycans, which not only contains biological cues for cell behavior, but is also a reservoir for binding growth factors and controlling their release. Here we aimed to create a suitable bony microenvironment with cell-derived ECM and biodegradable β-tricalcium phosphate (β-TCP). More specifically, we investigated whether the ECM produced by bone marrow-derived mesenchymal stem cells (hBMSC) on a β-TCP scaffold can bind bone morphogenetic protein-2 (BMP-2) and control its release in a sustained manner, and further examined the effect of ECM and the BMP-2 released from ECM on cell behaviors. The ECM was obtained through culturing the hBMSC on a β-TCP porous scaffold and performing decellularization and sterilization. SEM, XPS, FTIR, and immunofluorescent staining results indicated the presence of ECM on the β-TCP and the amount of ECM increased with the incubation time. BMP-2 was loaded onto the β-TCP with and without ECM by immersing the scaffolds in the BMP-2 solution. The loading and release kinetics of the BMP-2 on the β-TCP/ECM were significantly slower than those on the β-TCP. The β-TCP/ECM exhibited a sustained release profile of the BMP-2, which was also affected by the amount of ECM. This is probably because the β-TCP/ECM has different binding mechanisms with BMP-2. The β-TCP/ECM promoted cell proliferation. Furthermore, the BMP-2-loaded β-TCP/ECM stimulated reorganization of the actin cytoskeleton, increased expression of alkaline phosphatase and calcium deposition by the cells compared to those without BMP-2 loading and the β-TCP with BMP-2 loading.
Authors:
Yunqing Kang; Sungwoo Kim; Ali Khademhosseini; Yunzhi Yang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-05-31
Journal Detail:
Title:  Biomaterials     Volume:  32     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-07-05     Completed Date:  2011-11-23     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  6119-30     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Bone Morphogenetic Protein 2 / administration & dosage*
Calcium Phosphates / chemistry*
Cells, Cultured
Extracellular Matrix / chemistry*
Humans
Mesenchymal Stromal Cells / cytology*,  metabolism
Microscopy, Electron, Scanning
Photoelectron Spectroscopy
Spectroscopy, Fourier Transform Infrared
Tissue Engineering / methods*
Tissue Scaffolds / chemistry
Grant Support
ID/Acronym/Agency:
R01 AR057837/AR/NIAMS NIH HHS; R01 AR057837-01A1/AR/NIAMS NIH HHS; R01 DE021468/DE/NIDCR NIH HHS; R01 DE021468-01/DE/NIDCR NIH HHS; R01AR057837/AR/NIAMS NIH HHS; R01DE021468/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Bone Morphogenetic Protein 2; 0/Calcium Phosphates; 0/beta-tricalcium phosphate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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