Document Detail

Creatine phosphate inhibition of adenylate deaminase is mainly due to pyrophosphate.
MedLine Citation:
PMID:  216695     Owner:  NLM     Status:  MEDLINE    
Inhibition of rat skeletal muscle adenylate deaminase by creatine phosphate reported previously is due to inorganic pyrophosphate present as a contaminant in commercial preparations of creatine phosphate. This conclusion is based on the following evidence: a compound that inhibits adenylate deaminase can be separated from commercially prepared creatine phosphate by ion exchange chromatography; the inhibition by "creatine phosphate" and by the separated inhibitory compound is relieved by treatment with inorganic pyrophosphatase; inhibition by inorganic pyrophosphate is similar to that produced by unpurified creatine phosphate; and pyrophosphate is present in commercially available creatine phosphate in amounts sufficient to account for the inhibition. Some commercial preparations of creatine phosphate contain much less pyrophosphate than others; these preparations are only weakly inhibitory. Inorganic triphosphate is a more powerful inhibitor of the enzyme than pyrophosphate; it may also be present as a contaminant in creatine phosphate.
T J Wheeler; J M Lowenstein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  254     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1979 Mar 
Date Detail:
Created Date:  1979-04-28     Completed Date:  1979-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1484-6     Citation Subset:  IM    
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MeSH Terms
AMP Deaminase / antagonists & inhibitors*
Diphosphates / pharmacology*
Muscles / enzymology
Nucleotide Deaminases / antagonists & inhibitors*
Phosphocreatine / pharmacology*
Reg. No./Substance:
0/Diphosphates; 67-07-2/Phosphocreatine; EC 3.5.4.-/Nucleotide Deaminases; EC Deaminase

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