Document Detail


Cre-loxP-mediated bax gene activation reduces growth rate and increases sensitivity to chemotherapeutic agents in human gastric cancer cells.
MedLine Citation:
PMID:  10880019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dysregulation of apoptosis may be closely related to the development of cancer and its chemoresistance. Overexpression of Bax, an inducer of apoptosis, has led to increased cell death in a variety of cancer cell lines. In this study, we investigated the effect of Bax overexpression in two gastric cancer cell lines, MKN-28 and MKN-45, using a Cre-loxP-mediated inducible expression system. After induction of bax, both cell lines showed decreased proliferation, partially due to increased cell death. Furthermore, Bax-expressing MKN-28 cells were more sensitive to cisplatin. These results indicate that up-regulation of the bax gene may provide a novel strategy for the treatment of gastric cancer.
Authors:
K Komatsu; S Suzuki; T Shimosegawa; J I Miyazaki; T Toyota
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer gene therapy     Volume:  7     ISSN:  0929-1903     ISO Abbreviation:  Cancer Gene Ther.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-10-18     Completed Date:  2000-10-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9432230     Medline TA:  Cancer Gene Ther     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  885-92     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine (III), Tohoku University School of Medicine, Sendai, Japan. kkomatsu@doc.med.akita-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects,  genetics
Blotting, Northern
Cisplatin / pharmacology*
Combined Modality Therapy
DNA Primers / chemistry
Gene Expression / drug effects
Gene Expression Regulation
Gene Therapy / methods
Humans
Integrases / genetics*
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms / metabolism,  pathology,  therapy*
Transcriptional Activation
Transfection
Tumor Cells, Cultured
Viral Proteins*
bcl-2-Associated X Protein
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/BAX protein, human; 0/DNA Primers; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Viral Proteins; 0/bcl-2-Associated X Protein; 15663-27-1/Cisplatin; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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