| The C-terminal region of a Lys49 myotoxin mediates Ca2+ influx in C2C12 myotubes. | |
| | |
MedLine Citation:
|
PMID: 19835905 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Myotoxins are abundant components of snake venoms, being a significant public health problem worldwide. Among them, Lys49 phospholipase A(2) homologue myotoxins cause extensive necrosis in skeletal muscle tissue. Their mechanisms of action are still poorly understood, but there is evidence that the C-terminal region is involved in membrane damage leading to myotoxicity. To investigate the effect of the C-terminal peptide 115-129 of Agkistrodon contortrix laticinctus myotoxin on the plasma membrane of myoblasts and myotubes, the entry of Ca(2+) was monitored by fluorescence imaging, and the ensuing cytotoxicity was determined. The myotoxin synthetic peptide was found to act selectively on myotubes, which were rapidly overloaded with Ca(2+) with ensuing necrosis. The profile of intracellular Ca(2+) increase induced by the C-terminal peptide, but not by its scrambled version control, reproduces the second, prominent wave of the biphasic response documented in previous studies using whole Lys49 myotoxins. These observations provide relevant insights into the mechanism of action of this family of toxins, with implications for the understanding of their structure-function relationships. |
| | |
Authors:
|
Mariana Cintra-Francischinelli; Paola Pizzo; Yamileth Angulo; José M Gutiérrez; Cesare Montecucco; Bruno Lomonte |
Related Documents
:
|
15667845 - Nitrate toxicity to aquatic animals: a review with new data for freshwater invertebrates. 20386915 - Dietary b vitamin intakes and urinary total arsenic concentration in the health effects... 22321915 - Development and evaluation of a photographic atlas as a tool for dietary assessment stu... 301255 - Microscopic effects of meglumine iothalamate. ventriculography in canines. 3535815 - Role of dietary and environmental factors in drug abuse. 20161605 - The association between membership in the sandwich generation and health behaviors: a l... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-14 |
Journal Detail:
|
Title: Toxicon : official journal of the International Society on Toxinology Volume: 55 ISSN: 1879-3150 ISO Abbreviation: Toxicon Publication Date: 2010 Feb-Mar |
Date Detail:
|
Created Date: 2010-01-29 Completed Date: 2010-04-08 Revised Date: 2011-11-24 |
Medline Journal Info:
|
Nlm Unique ID: 1307333 Medline TA: Toxicon Country: England |
Other Details:
|
Languages: eng Pagination: 590-6 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Dipartimento di Scienze Biomediche, Universitá di Padova, Padova, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Agkistrodon Animals Calcium / metabolism* Cell Line Cell Survival / drug effects Mice Muscle Fibers, Skeletal / drug effects, metabolism* Muscle, Skeletal / drug effects, metabolism Myoblasts / drug effects, metabolism Peptides / chemistry, toxicity Phospholipases A2 / toxicity* Structure-Activity Relationship Viper Venoms / enzymology, toxicity* |
| Grant Support | |
ID/Acronym/Agency:
|
GGP06133//Telethon |
| Chemical | |
Reg. No./Substance:
|
0/Peptides; 0/Viper Venoms; 7440-70-2/Calcium; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sensitivity of lateral flow tests to mixtures of saxitoxins and applications to shellfish and phytop...
Next Document: Biological and Proteomic Analysis of Venom from the Puerto Rican Racer (Alsophis portoricensis: Dips...