| Craniopharyngiomas express embryonic stem cell markers (SOX2, OCT4, KLF4, and SOX9) as pituitary stem cells but do not coexpress RET/GFRA3 receptors. | |
| | |
MedLine Citation:
|
PMID: 22031517 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
CONTEXT: Adult stem cells maintain some markers expressed by embryonic stem cells and express other specific markers depending on the organ where they reside. Recently, stem/progenitor cells in the rodent and human pituitary have been characterized as expressing GFRA2/RET, PROP1, and stem cell markers such as SOX2 and OCT4 (GPS cells). OBJECTIVE: Our objective was to detect other specific markers of the pituitary stem cells and to investigate whether craniopharyngiomas (CRF), a tumor potentially derived from Rathke's pouch remnants, express similar markers as normal pituitary stem cells. DESIGN: We conducted mRNA and Western blot studies in pituitary extracts, and immunohistochemistry and immunofluorescence on sections from normal rat and human pituitaries and 20 CRF (18 adamantinomatous and two papillary). RESULTS: Normal pituitary GPS stem cells localized in the marginal zone (MZ) express three key embryonic stem cell markers, SOX2, OCT4, and KLF4, in addition to SOX9 and PROP1 and β-catenin overexpression. They express the RET receptor and its GFRA2 coreceptor but also express the coreceptor GFRA3 that could be detected in the MZ of paraffin pituitary sections. CRF maintain the expression of SOX2, OCT4, KLF4, SOX9, and β-catenin. However, RET and GFRA3 expression was altered in CRF. In 25% (five of 20), both RET and GFRA3 were detected but not colocalized in the same cells. The other 75% (15 of 20) lose the expression of RET, GFRA3, or both proteins simultaneously. CONCLUSIONS: Human pituitary adult stem/progenitor cells (GPS) located in the MZ are characterized by expression of embryonic stem cell markers SOX2, OCT4, and KLF4 plus the specific pituitary embryonic factor PROP1 and the RET system. Redundancy in RET coreceptor expression (GFRA2 and GFRA3) suggest an important systematic function in their physiological behavior. CRF share the stem cell markers suggesting a common origin with GPS. However, the lack of expression of the RET/GFRA system could be related to the cell mislocation and deregulated growth of CRF. |
| | |
Authors:
|
Montserrat Garcia-Lavandeira; Carmen Saez; Esther Diaz-Rodriguez; Sihara Perez-Romero; Ana Senra; Carlos Dieguez; Miguel A Japon; Clara V Alvarez |
Related Documents
:
|
22084067 - Organ aging and susceptibility to cancer may be related to the geometry of the stem cel... 10994637 - A method for the dispersal and characterization of leukocytes from the human female rep... 12876667 - Clinical features of large granular lymphocyte leukemia. 21904387 - Inflammatory modulation of hscs: viewing the hsc as a foundation for the immune response. 8725877 - Transduction of human renal carcinoma cells with human gamma-interferon gene via retrov... 21350367 - The effect of intermittent il-2 therapy on cd4 t cells in the gut in hiv-1-infected pat... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-10-26 |
Journal Detail:
|
Title: The Journal of clinical endocrinology and metabolism Volume: 97 ISSN: 1945-7197 ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-01-06 Completed Date: 2012-02-27 Revised Date: 2012-06-04 |
Medline Journal Info:
|
Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: United States |
Other Details:
|
Languages: eng Pagination: E80-7 Citation Subset: AIM; IM |
Affiliation:
|
Instituto de Investigaciones Sanitarias & Centro de Investigaciones Médicas de la USC (IDIS-CIMUS), Department of Physiology, School of Medicine, University of Santiago de Compostela (USC), E-15782 Santiago de Compostela, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Adult Stem Cells / metabolism* Animals Biological Markers / metabolism* Craniopharyngioma / genetics*, metabolism, pathology Embryonic Stem Cells / metabolism* Gene Expression Regulation, Neoplastic Glial Cell Line-Derived Neurotrophic Factor Receptors / genetics*, metabolism Humans Kruppel-Like Transcription Factors / genetics, metabolism Male Octamer Transcription Factor-3 / genetics, metabolism Pituitary Gland / cytology, metabolism Pituitary Neoplasms / genetics*, metabolism, pathology Proto-Oncogene Proteins c-ret / genetics*, metabolism Rats Rats, Wistar SOX9 Transcription Factor / genetics, metabolism SOXB1 Transcription Factors / genetics, metabolism Tumor Markers, Biological / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/GFRA3 protein, human; 0/GKLF protein; 0/Glial Cell Line-Derived Neurotrophic Factor Receptors; 0/Kruppel-Like Transcription Factors; 0/Octamer Transcription Factor-3; 0/POU5F1 protein, human; 0/SOX2 protein, human; 0/SOX9 Transcription Factor; 0/SOX9 protein, human; 0/SOXB1 Transcription Factors; 0/Tumor Markers, Biological; EC 2.7.10.1/Proto-Oncogene Proteins c-ret; EC 2.7.10.1/RET protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Heterogeneity of Focal Forms of Congenital Hyperinsulinism.
Next Document: High-Dose Insulin Therapy Reduces Postoperative Liver Dysfunction and Complications in Liver Resecti...