| CrATP interferes in the promastigote-macrophage interaction in Leishmania amazonensis infection. | |
| | |
MedLine Citation:
|
PMID: 21679488 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
SUMMARYRecent have shown the relationship between Ecto-Nucleoside-Triphosphate-Diphosphohydrolases (Ecto-NTPDases or ecto-nucleotidases) and virulence and infectivity in trypanosomatids. In this work, the inhibition of the ecto-ATPase activities and promastigote growth of Leishmania amazonensis by CrATP was characterized. Furthermore, this compound was used to investigate the role of ecto-nucleotidase in the interaction of L. amazonensis with resident peritoneal macrophages obtained from BALB/c mice. CrATP partially inhibits the ecto-ATPase activity, presenting Ki values of 575·7±199·1 and 383·5±79·0 μm, in the presence or absence of 5 mm MgCl2, respectively. The apparent Kms for ATP (2·9±0·5 mm to Mg2+-dependent ecto-ATPase and 0·4±0·2 mm to Mg2+-independent ecto-ATPase activities) are not significantly altered by CrATP, suggesting a reversible non-competitive inhibition of both enzymes. When CrATP was added to the cultivation medium at 500 μm, it drastically inhibited the cellular growth. The interaction of promastigote forms of L. amazonensis with BALB/c peritoneal macrophages is strongly affected by CrATP. When the parasites were treated with 500 μm CrATP before interacting with macrophages, the adhesion and endocytic indices were strongly reduced to 53·0±14·8% and 39·8±1·1%, respectively. These results indicate that ecto-nucleotidase plays an important role in the infection process caused by Leishmania amazonensis. |
| | |
Authors:
|
V Ennes-Vidal; R O S Castro; C Britto; H Barrabin; C M D'Avila-Levy; O C Moreira |
Related Documents
:
|
17422948 - Adjuvants et stimulants de l'immunité: propriétés immunorégulatrices du muramyl-dip... 19524428 - Functional implications of t cell receptor diversity. 19068548 - The roles of cathelicidin ll-37 in immune defences and novel clinical applications. 15180158 - Histopathology of sclerotinia sclerotiorum attack on flower parts of helianthus annuus ... 23487828 - Neurological immune-related adverse events of ipilimumab. 21547348 - Imiquimod and lymphatic field clearance: a new hypothesis based on a remote immune acti... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-17 |
Journal Detail:
|
Title: Parasitology Volume: - ISSN: 1469-8161 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0401121 Medline TA: Parasitology Country: - |
Other Details:
|
Languages: ENG Pagination: 1-9 Citation Subset: - |
Affiliation:
|
Laboratório de Biologia Molecular e Doenças Endêmicas, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Towards a new role for vector systematics in parasite control.
Next Document: Population genetics of Schistosoma haematobium: development of novel microsatellite markers and thei...