| Cpkk1, MAPKK of Cryphonectria parasitica, is necessary for virulence on chestnut. | |
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MedLine Citation:
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PMID: 20839945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ABSTRACT The role of Cpkk1, a mitogen-activated protein kinase from Cryphonectria parasitica, was investigated by generating a number of mutant strains that overexpress, under the control of the cryparin promoter, both the wild-type protein and its allele with an extensive deletion in the catalytic domain. Furthermore, a hairpin construct was built and expressed to cause specific silencing of Cpkk1 mRNA transcripts. Specific mRNA silencing or overexpression was confirmed on both Northern and Western blot analysis. Selected C. parasitica strains with Cpkk1 either silenced or overexpressed were evaluated for their biological characteristics, including virulence on European chestnut, growth on different substrates, conidial sporulation, and resistance to cell-wall-degrading enzymes. Silencing of Cpkk1 and the overexpression of a defective Cpkk1 correlated with a marked reduction in virulence on 3-year-old chestnut trees, with no statistically significant effect on fungal growth in the various conditions tested. |
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Authors:
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Laura Rostagno; Antonio Prodi; Massimo Turina |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Phytopathology Volume: 100 ISSN: 0031-949X ISO Abbreviation: Phytopathology Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-15 Completed Date: 2010-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9427222 Medline TA: Phytopathology Country: United States |
Other Details:
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Languages: eng Pagination: 1100-10 Citation Subset: IM |
Affiliation:
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Istituto di Virologia Vegetale, CNR, Torino, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aesculus
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microbiology* Ascomycota / enzymology*, genetics*, pathogenicity Fungal Proteins / genetics, metabolism* Gene Expression Regulation, Fungal Gene Silencing Plant Diseases / microbiology* RNA, Fungal / genetics, metabolism RNA, Messenger / genetics, metabolism Virulence |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/RNA, Fungal; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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