Document Detail

Cpkk1, MAPKK of Cryphonectria parasitica, is necessary for virulence on chestnut.
MedLine Citation:
PMID:  20839945     Owner:  NLM     Status:  MEDLINE    
ABSTRACT The role of Cpkk1, a mitogen-activated protein kinase from Cryphonectria parasitica, was investigated by generating a number of mutant strains that overexpress, under the control of the cryparin promoter, both the wild-type protein and its allele with an extensive deletion in the catalytic domain. Furthermore, a hairpin construct was built and expressed to cause specific silencing of Cpkk1 mRNA transcripts. Specific mRNA silencing or overexpression was confirmed on both Northern and Western blot analysis. Selected C. parasitica strains with Cpkk1 either silenced or overexpressed were evaluated for their biological characteristics, including virulence on European chestnut, growth on different substrates, conidial sporulation, and resistance to cell-wall-degrading enzymes. Silencing of Cpkk1 and the overexpression of a defective Cpkk1 correlated with a marked reduction in virulence on 3-year-old chestnut trees, with no statistically significant effect on fungal growth in the various conditions tested.
Laura Rostagno; Antonio Prodi; Massimo Turina
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytopathology     Volume:  100     ISSN:  0031-949X     ISO Abbreviation:  Phytopathology     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-15     Completed Date:  2010-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9427222     Medline TA:  Phytopathology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1100-10     Citation Subset:  IM    
Istituto di Virologia Vegetale, CNR, Torino, Italy.
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MeSH Terms
Aesculus / microbiology*
Ascomycota / enzymology*,  genetics*,  pathogenicity
Fungal Proteins / genetics,  metabolism*
Gene Expression Regulation, Fungal
Gene Silencing
Plant Diseases / microbiology*
RNA, Fungal / genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Reg. No./Substance:
0/Fungal Proteins; 0/RNA, Fungal; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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