| CpG oligonucleotides induce an immune response of odontoblasts through the TLR9, MyD88 and NF-kappaB pathways. | |
| | |
MedLine Citation:
|
PMID: 20655295 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Odontoblasts are the first-line defense cells against invading microorganisms. Toll-like receptors (TLRs) play a crucial role in innate immunity, and TLR9 is involved in the recognition of microbial DNA. This study aimed to investigate whether odontoblasts can respond to CpG DNA and to determine the intracellular signaling pathways triggered by CpG DNA. We found that the mouse odontoblast-like cell line MDPC-23 constitutively expressed TLR9. Exposure to CpG ODN induced a potent proinflammatory response based on an increase of IL-6 and TNF-alpha expression. Pretreatment with an inhibitory MyD88 peptide or a specific inhibitor for TLR9, NF-kappaB or IkappaBalpha markedly inhibited CpG ODN-induced IL-6 and TNF-alpha expression. Moreover, the CpG ODN-mediated increase of kappaB-luciferase activity in MDPC-23 cells was suppressed by the overexpression of dominant negative mutants of TLR9, MyD88 and IkappaBalpha, but not by the dominant negative mutant of TLR4. This result suggests a possible role for the CpG DNA-mediated immune response in odontoblasts and indicates that TLR9, MyD88 and NF-kappaB are involved in this process. |
| | |
Authors:
|
Wenxi He; Qing Yu; Zeyuan Zhou; Ping Wang |
Related Documents
:
|
18990205 - The role of interleukin-12 in the heavy metal-elicited immunomodulation: relevance of v... 17457675 - A multi-scale model of dendritic cell education and trafficking in the lung: implicatio... 20202485 - Administered cpg oligodeoxynucleotide induces mrna expression of cxc and cc chemokines ... 19896155 - All three classes of cpg odns up-regulate ip-10 gene in pigs. 7509225 - C-kit ligand augments granulocyte-macrophage colony growth from patients with 5q- syndr... 12496405 - Efficient induction of primary and secondary t cell-dependent immune responses in vivo ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-21 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 399 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-23 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 274-8 Citation Subset: IM |
Copyright Information:
|
Crown Copyright 2010. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Operative Dentistry and Endodontics, School of Stomatology, Fourth Military Medical University (FMMU), Xi'an 710032, PR China. hewenxi@fmmu.edu.cn |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line CpG Islands / immunology* Cytokines / biosynthesis DNA, Bacterial / immunology* Mice Myeloid Differentiation Factor 88 / metabolism* NF-kappa B / metabolism* Odontoblasts / drug effects, immunology* Oligodeoxyribonucleotides / immunology, pharmacology Toll-Like Receptor 9 / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/CpG ODN 2006; 0/Cytokines; 0/DNA, Bacterial; 0/Myd88 protein, mouse; 0/Myeloid Differentiation Factor 88; 0/NF-kappa B; 0/Oligodeoxyribonucleotides; 0/Tlr9 protein, mouse; 0/Toll-Like Receptor 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: IM-412 Inhibits Transforming Growth Factor beta-induced Fibroblast Differentiation in Human Lung Fib...
Next Document: Fusion protein based on Grb2-SH2 domain for cancer therapy.