| CpG oligodeoxynucleotide and double-stranded RNA synergize to enhance nitric oxide production and mRNA expression of inducible nitric oxide synthase, pro-inflammatory cytokines and chemokines in chicken monocytes. | |
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MedLine Citation:
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PMID: 20083501 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Toll-like receptors (TLRs) recognize microbial components and initiate the innate immune responses that control microbial infections. The interaction between ligands of TLR3 and TLR9, poly I:C (an analog of viral double-stranded RNA) and CpG-ODN (a CpG-motif containing oligodeoxydinucleotide) on the inflammatory immune responses, including the production of nitric oxide (NO) and the expression of inducible NO synthase (iNOS), pro-inflammatory cytokines interleukin (IL)-1β and IL-6, and chemokines IL-8 and macrophage inflammatory protein (MIP)-1β, were investigated in chicken monocytes. The NO production was significantly higher when stimulated with a combination of CpG-ODN and poly I:C than with either CpG-ODN or poly I:C alone. Similarly, a significant synergistic effect by CpG-ODN and poly I:C was observed in the up-regulation of iNOS and IL-8 mRNA after 2 h and persisted up to 24 h. Although the combinatory treatment of CpG-ODN and poly I:C enhanced the expression of IL-1β, IL-6, and MIP-1β(3 after 2 h stimulation, the synergism in the up-regulation of IL-1β and IL-6 mRNA was observed after 8-h and 24-h stimulation, respectively, whereas there was no synergistic effect on MIP-1β. Our results demonstrate that CpG-ODN synergizes with poly I:C to induce pro-inflammatory immune response in chicken monocytes. |
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Authors:
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Haiqi He; Kathryn M MacKinnon; Kenneth J Genovese; Michael H Kogut |
Publication Detail:
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Type: Journal Article Date: 2010-01-18 |
Journal Detail:
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Title: Innate immunity Volume: 17 ISSN: 1753-4267 ISO Abbreviation: Innate Immun Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-05 Completed Date: 2011-07-28 Revised Date: 2011-09-14 |
Medline Journal Info:
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Nlm Unique ID: 101469670 Medline TA: Innate Immun Country: United States |
Other Details:
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Languages: eng Pagination: 137-44 Citation Subset: IM |
Affiliation:
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Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, College Station, Texas, USA. haiqi.he@ars.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Avian Proteins / agonists Cells, Cultured Chickens Cytokines / genetics, metabolism Drug Synergism Inflammation Mediators / metabolism Leukocytes, Mononuclear / drug effects, immunology, metabolism*, pathology Nitric Oxide / metabolism Nitric Oxide Synthase Type II / genetics, immunology, metabolism* Oligodeoxyribonucleotides / pharmacology* Poly I-C / pharmacology RNA, Double-Stranded / pharmacology* Receptor Cross-Talk* Toll-Like Receptors / agonists |
| Chemical | |
Reg. No./Substance:
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0/Avian Proteins; 0/CPG-oligonucleotide; 0/Cytokines; 0/Inflammation Mediators; 0/Oligodeoxyribonucleotides; 0/RNA, Double-Stranded; 0/Toll-Like Receptors; 10102-43-9/Nitric Oxide; 24939-03-5/Poly I-C; EC 1.14.13.39/Nitric Oxide Synthase Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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