| CpG and LPS can interfere negatively with prion clearance in macrophage and microglial cells. | |
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MedLine Citation:
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PMID: 17944938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells of the innate immune system play important roles in the progression of prion disease after peripheral infection. It has been found in vivo and in vitro that the expression of the cellular prion protein (PrP(c)) is up-regulated on stimulation of immune cells, also indicating the functional importance of PrP(c) in the immune system. The aim of our study was to investigate the impact of cytosine-phosphate-guanosine- and lipopolysaccharide-induced PrP(c) up-regulation on the uptake and processing of the pathological prion protein (PrP(Sc)) in phagocytic innate immune cells. For this purpose, we challenged the macrophage cell line J774, the microglial cell line BV-2 and primary bone marrow-derived macrophages in a resting or stimulated state with various prion strains, and monitored the uptake and clearance of PrP(Sc). Interestingly, stimulation led either to a transient increase in the level of PrP(Sc) relative to unstimulated cells or to a decelerated degradation of PrP(Sc). These features were dependent on cell type and prion strain. Our data indicate that the stimulation of innate immune cells may be able to support transient prion propagation, possibly explained by an increased PrP(c) cell surface expression in stimulated cells. We suggest that stimulation of innate immune cells can lead to an imbalance between the propagation and degradation of PrP(Sc). |
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Authors:
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Sabine Gilch; Frank Schmitz; Yasmine Aguib; Claudia Kehler; Sigrid Bülow; Stefan Bauer; Elisabeth Kremmer; Hermann M Schätzl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-18 |
Journal Detail:
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Title: The FEBS journal Volume: 274 ISSN: 1742-464X ISO Abbreviation: FEBS J. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-06 Completed Date: 2008-01-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101229646 Medline TA: FEBS J Country: England |
Other Details:
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Languages: eng Pagination: 5834-44 Citation Subset: IM |
Affiliation:
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Institute of Virology, Prion Research Group, Technical University of Munich, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals CpG Islands* Flow Cytometry Lipopolysaccharides / pharmacology* Macrophages / metabolism* Mice Mice, Inbred Strains Microglia / metabolism* Prions / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/Prions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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