| Coxsackievirus B3-induced apoptosis and caspase-3. | |
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MedLine Citation:
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PMID: 12862321 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell death can be classified into two categories: apoptosis and necrosis. Apoptotic pathway can be either caspase-dependent or caspase-independent. Caspase-independent cytopathic effect (CPE) has been described. In order to evaluate the pattern of HeLa cell death induced by Coxsackievirus B3 (CVB3) and whether apoptosis involves caspase activation, we co-cultivated HeLa cells with CVB3 and detected the cytopathic changes, the alteration of mRNA and protein expression of caspase-3 gene plus caspase-3 activity, as well as analyzing DNA fragmentation before and after caspase-3 activity inhibition. According to the results, we propose that CVB3 may induce apoptosis and necrosis in HeLa cells, the latter appearing much earlier. Caspase-3 is activated at the levels of both transcription and translation, and procaspase-3 is proteolytically cleaved, thus leading to the continuous increasing of both caspase-3 precursor protein and its subunit. However, besides CPE, apoptosis induced by CVB3 is not a direct consequence of the activation of caspase-3, or caspase-3 is not the only effector molecule in apoptotic cell death, for caspase-3 inhibitor can not decrease DNA fragmentation. Some other biochemical mechanisms may participate in the process, whose role weakens the effect of inhibiting caspase-3 activity. |
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Authors:
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Jian Ping Yuan; Wei Zhao; Hong Tao Wang; Kai Yu Wu; Tao Li; Xiao Kui Guo; Shan Qing Tong |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell research Volume: 13 ISSN: 1001-0602 ISO Abbreviation: Cell Res. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-07-16 Completed Date: 2004-02-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9425763 Medline TA: Cell Res Country: China |
Other Details:
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Languages: eng Pagination: 203-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Parasitology, Shanghai Second Medical University, Shanghai 200025, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
physiology* Caspase 3 Caspases / antagonists & inhibitors, genetics, metabolism* DNA Fragmentation / drug effects Enterovirus B, Human / physiology* Enzyme Activation / drug effects Enzyme Inhibitors / pharmacology Flow Cytometry Hela Cells / ultrastructure, virology Humans Microscopy, Electron Oligopeptides / pharmacology Protein Biosynthesis / genetics RNA, Messenger / metabolism Time Factors Transcription, Genetic / genetics |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Oligopeptides; 0/RNA, Messenger; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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