Document Detail

Coxsackie B1 virus-induced changes in cell membrane-associated functions are not responsible for altered sensitivity to bacterial invasiveness.
MedLine Citation:
PMID:  1318709     Owner:  NLM     Status:  MEDLINE    
To analyze the possible mechanisms by which coxsackie B1 virus infection affects the invasiveness of Shigella flexneri, we have studied the influence of intracellular levels of Na+ and K+, ATPase activity, cytoplasmic membrane potential, cAMP level and cell communication through gap junctions. 3h after adsorption of viable or UV-inactivated coxsackie B1 virus the Na(+)-K+ gradient of the cell collapsed, ATPase activity decreased, the cytoplasmic membranic potential-dependent tetraphosphonium ion uptake were reduced. No changes in cAMP or intercellular cell communication were observed. S. flexneri invasiveness in HEp-2 cell pretreated with viable or UV-inactivated coxsackie B 1 virus was enhanced, but bacterial invasiveness was unchanged in K(+)-depleted HEp-2 cells, cell cultures with high intracellular Na+ content or ouabain pre-treated cells compared to control cells. We found no correlation between the enhanced bacterial invasiveness in the early phase of coxsackie B 1 virus infection in HEp-2 cell cultures and intracellular K+ depletion, high intracellular Na+ content, inhibited Na(+)-K+ ATPase activity or membranic depolarization.
K Modalsli; G Bukholm; S O Mikalsen; M Degré
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of virology     Volume:  124     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  1992  
Date Detail:
Created Date:  1992-07-14     Completed Date:  1992-07-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  AUSTRIA    
Other Details:
Languages:  eng     Pagination:  321-32     Citation Subset:  IM    
Kaptein W. Wilhelmsen og Frues Bakteriologiske Institutt, University of Oslo, Norway.
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MeSH Terms
Cell Communication
Cell Line
Cell Membrane / microbiology*,  physiology
Cyclic AMP / metabolism
Enterovirus B, Human / physiology*,  radiation effects
Intercellular Junctions / metabolism
Membrane Potentials
Onium Compounds / metabolism
Organophosphorus Compounds / metabolism
Ouabain / pharmacology
Potassium / metabolism
Shigella flexneri / physiology*
Signal Transduction
Sodium / metabolism
Sodium-Potassium-Exchanging ATPase / metabolism
Ultraviolet Rays
Reg. No./Substance:
0/Onium Compounds; 0/Organophosphorus Compounds; 18198-39-5/tetraphenylphosphonium; 60-92-4/Cyclic AMP; 630-60-4/Ouabain; 7440-09-7/Potassium; 7440-23-5/Sodium; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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