Document Detail

Cow's milk challenge through human milk evokes immune responses in infants with cow's milk allergy.
MedLine Citation:
PMID:  10518086     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: In order to measure the immune response evoked in breast-fed infants with cow's milk allergy (CMA) by cow's milk challenge through human milk, mothers were given increasing doses of cow's milk after they had been on a cow's milk elimination diet. Another objective was to study the secretion of beta-lactoglobulin (BLG) into human milk before and during milk challenge in relation to the appearance of symptoms in infants. STUDY DESIGN: Seventeen asymptomatic mothers who had infants with challenge-proven CMA and 10 asymptomatic mothers who had healthy infants were recruited. Infants ranged in age from 1.8 to 9.4 months. A solid-phase enzyme-linked immunoassay (ELISPOT) was used to assess the total number of immunoglobulin-secreting and specific antibody-secreting cells. Flow cytometry was used to enumerate different lymphocyte subpopulations among peripheral blood lymphocytes primed during provocation by cow's milk antigens. BLG levels were assessed in human milk before the challenge and 1, 2, 3, and 4 hours after the commencement of the challenge. RESULTS: All but one of the infants with CMA showed symptoms of CMA during cow's milk challenge through human milk. There was a significant rise in the total number of immunoglobulin-secreting cells in the IgA and IgG classes associated with a positive cow's milk challenge response, but the proportions of peripheral blood B cells bearing CD19, CD23, CD19 and 23, CD5, or CD19 and CD5 were comparable. BLG levels were comparable in both study groups. CONCLUSIONS: Most of the infants with CMA reacted to cow's milk challenge through human milk. Hypersensitivity reactions to food antigens through human milk may be more common than previously thought.
K M Järvinen; S Mäkinen-Kiljunen; H Suomalainen
Related Documents :
24746116 - Applying the icf-cy framework to examine biological and environmental factors in early ...
7115236 - Effect of calving supervision upon the calving performance of hereford heifers.
16650046 - Transforming growth factor-beta and interleukin-10 in breast milk and development of at...
3988656 - Growth patterns of cows sired by british and continental beef and american dairy bulls ...
12557066 - Three-dimensional volume measurement of the lateral ventricles in preterm and term infa...
11075086 - Multidisciplinary discharge assessment of the medically and socially high-risk infant.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pediatrics     Volume:  135     ISSN:  0022-3476     ISO Abbreviation:  J. Pediatr.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-04     Completed Date:  1999-11-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375410     Medline TA:  J Pediatr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  506-12     Citation Subset:  AIM; IM    
Helsinki University Central Hospital, Department of Dermatology, Helsinki, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
B-Lymphocyte Subsets
Breast Feeding*
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Lactoglobulins / analysis
Milk / immunology
Milk Hypersensitivity / diagnosis*,  etiology,  immunology
Milk, Human / chemistry
Prospective Studies
Skin Tests
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Diversity in presenting manifestations of systemic lupus erythematosus in children.
Next Document:  Characterization of esophageal body and lower esophageal sphincter motor function in the very premat...