| Cowpea mosaic virus RNA-1 acts as an amplicon whose effects can be counteracted by a RNA-2-encoded suppressor of silencing. | |
| | |
MedLine Citation:
|
PMID: 15165817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lines of Nicotiana benthamiana transgenic for full-length copies of both Cowpea mosaic virus (CPMV) genomic RNAs, either singly or together, have been produced. Plants transgenic for both RNAs developed symptoms characteristic of a CPMV infection. When plants transgenic for RNA-1 were agro-inoculated with RNA-2, no infection developed and the plants were also resistant to challenge with CPMV. By contrast, plants transgenic for RNA-2 became infected when agro-inoculated with RNA-1 and were fully susceptible to CPMV infection. The resistance of RNA-1 transgenic plants was shown to be related to the ability of RNA-1 to self-replicate and act as an amplicon. The ability of transgenically expressed RNA-2 to counteract the amplicon effect suggested that it encodes a suppressor of posttranscriptional gene silencing (PTGS). By examining the ability of portions of RNA-2 to reverse PTGS in N. benthamiana, we have identified the small (S) coat protein as the CPMV RNA-2-encoded suppressor of PTGS. |
| | |
Authors:
|
Li Liu; Jef Grainger; M Carmen Cañizares; Susan M Angell; George P Lomonossoff |
Related Documents
:
|
23708527 - Gene expression profiling as functional readout of rodent models for psychiatric disord... 19825657 - Expression of pin genes in rice (oryza sativa l.): tissue specificity and regulation by... 7773307 - Functional expression of saccharomyces cerevisiae cyp51a1 encoding lanosterol-14-demeth... 11463167 - Molecular basis of the anaerobic response in plants. 7707957 - Isolation and analysis of microtubule motor proteins. 10467397 - Isolation of dice1: a gene frequently affected by loh and downregulated in lung carcino... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Virology Volume: 323 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2004 May |
Date Detail:
|
Created Date: 2004-05-28 Completed Date: 2004-07-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
|
Languages: eng Pagination: 37-48 Citation Subset: IM |
Affiliation:
|
John Innes Centre, Colney Lane, Norwich NR4 7UH, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Capsid Proteins
/
genetics,
metabolism Comovirus / genetics*, physiology* Genetic Complementation Test Plant Diseases / virology* Plant Leaves / genetics, virology Plant Viral Movement Proteins Plants, Genetically Modified / genetics, virology RNA Interference* RNA, Viral / genetics*, metabolism Tobacco / genetics, virology Viral Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Capsid Proteins; 0/Plant Viral Movement Proteins; 0/RNA, Viral; 0/Viral Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interspecies transmission of an H7N3 influenza virus from wild birds to intensively reared domestic ...
Next Document: The multifunctional plant viral suppressor of gene silencing P19 interacts with itself and an RNA bi...