Document Detail

Cowpea mosaic virus RNA-1 acts as an amplicon whose effects can be counteracted by a RNA-2-encoded suppressor of silencing.
MedLine Citation:
PMID:  15165817     Owner:  NLM     Status:  MEDLINE    
Lines of Nicotiana benthamiana transgenic for full-length copies of both Cowpea mosaic virus (CPMV) genomic RNAs, either singly or together, have been produced. Plants transgenic for both RNAs developed symptoms characteristic of a CPMV infection. When plants transgenic for RNA-1 were agro-inoculated with RNA-2, no infection developed and the plants were also resistant to challenge with CPMV. By contrast, plants transgenic for RNA-2 became infected when agro-inoculated with RNA-1 and were fully susceptible to CPMV infection. The resistance of RNA-1 transgenic plants was shown to be related to the ability of RNA-1 to self-replicate and act as an amplicon. The ability of transgenically expressed RNA-2 to counteract the amplicon effect suggested that it encodes a suppressor of posttranscriptional gene silencing (PTGS). By examining the ability of portions of RNA-2 to reverse PTGS in N. benthamiana, we have identified the small (S) coat protein as the CPMV RNA-2-encoded suppressor of PTGS.
Li Liu; Jef Grainger; M Carmen Cañizares; Susan M Angell; George P Lomonossoff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  323     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-28     Completed Date:  2004-07-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-48     Citation Subset:  IM    
John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
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MeSH Terms
Capsid Proteins / genetics,  metabolism
Comovirus / genetics*,  physiology*
Genetic Complementation Test
Plant Diseases / virology*
Plant Leaves / genetics,  virology
Plant Viral Movement Proteins
Plants, Genetically Modified / genetics,  virology
RNA Interference*
RNA, Viral / genetics*,  metabolism
Tobacco / genetics,  virology
Viral Proteins / genetics,  metabolism
Reg. No./Substance:
0/Capsid Proteins; 0/Plant Viral Movement Proteins; 0/RNA, Viral; 0/Viral Proteins

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