Document Detail

Cowdria ruminantium identified in Amblyomma gemma using a DNA probe pCS20.
MedLine Citation:
PMID:  8134629     Owner:  NLM     Status:  MEDLINE    
Amblyomma gemma ticks were collected from wild animals on a 20,000 acre game ranch in a heartwater endemic area in Kenya, close to Nairobi. A. variegatum is the main vector of heartwater in Kenya. E.A. LEWIS, 1947, in a one sentence report has implicated A. gemma to be a vector of heartwater without giving any details. Adult A. gemma were collected from giraffe Giraffa camelopardalis, hartebeest Alcephalus busephalus, Eland Taurotragus oryx and ostrich Struthio camelus during cropping exercises. The unengorged ticks were fed on 3 susceptible Dorper sheep which were monitored daily for the clinical symptoms of heartwater. All the ticks, including those that were fed on sheep were dissected and the guts probed for the presence of Cowdria ruminantium using a cloned DNA probe, the pCS20. None of the sheep on which the ticks were fed showed heartwater symptoms up to 60 days from the attachment of the ticks. The DNA probe identified Cowdria ruminantium in the ticks collected from eland and giraffe.
F D Wesonga; S W Mukolwe; F Rurangirwa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Revue d'élevage et de médecine vétérinaire des pays tropicaux     Volume:  46     ISSN:  0035-1865     ISO Abbreviation:  Rev Elev Med Vet Pays Trop     Publication Date:  1993  
Date Detail:
Created Date:  1994-04-20     Completed Date:  1994-04-20     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2984776R     Medline TA:  Rev Elev Med Vet Pays Trop     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  179-81     Citation Subset:  IM    
Kenya Agricultural Research Institute, Uthiru, Nairobi.
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MeSH Terms
DNA Probes
Ehrlichia ruminantium / isolation & purification*
Heartwater Disease / transmission
Ticks / microbiology*
Reg. No./Substance:
0/DNA Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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