| Covalent immobilization of invertase and horseradish peroxidase on concanavalin A-Seralose via carbohydrate moieties. | |
| | |
MedLine Citation:
|
PMID: 7549967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Invertase from Baker's yeast (Saccharomyces cerevisiae) and Horseradish peroxidase (HRP) were covalently immobilized on Concanavalin A precoupled to Seralose via carbohydrate moieties. Covalent coupling of glycoenzymes was achieved by periodate induced aldehydic groups of glycosyls with amino groups of Concanavalin A, at different pH values. A bifunctional reagent such as glutaraldehyde crosslinks the glycoenzymes with lectin both intra and intermolecularly. Therefore, attempts were made to introduce covalent linkages between glycoenzymes and Concanavalin A-Seralose without intramolecular crosslinking in either. The immobilized preparations of glycoenzymes exhibited high yield of immobilization and eta value. About 90 and 85% covalent coupling could be observed in invertase and HRP at pH 7.0 respectively, as determined by treatment with 0.5 M methyl alpha-D-mannopyranoside. All immobilized glycoenzyme preparations exhibited marked stabilization towards thermal inactivation. |
| | |
Authors:
|
S Husain; F Jafri |
Related Documents
:
|
5438767 - A survey of hemoglobin values in canada. 1156307 - Probability of casualties in an airport disaster. 7616987 - Sce analysis in human lymphocytes of a spanish control population. 15328397 - Physical and chemical compatibility of daptomycin with nine medications. 11197927 - Factors associated with immediate dropout of outpatient treatment for drug abuse in rio... 12429077 - Menstrual nirvana: amenorrhea through the use of continuous oral contraceptives. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochemistry and molecular biology international Volume: 36 ISSN: 1039-9712 ISO Abbreviation: Biochem. Mol. Biol. Int. Publication Date: 1995 Jul |
Date Detail:
|
Created Date: 1995-11-22 Completed Date: 1995-11-22 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9306673 Medline TA: Biochem Mol Biol Int Country: AUSTRALIA |
Other Details:
|
Languages: eng Pagination: 669-77 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carbohydrates Concanavalin A Enzymes, Immobilized* Glycoside Hydrolases* Hydrogen-Ion Concentration Peroxidase* Plant Lectins Plants / enzymology Saccharomyces cerevisiae / enzymology beta-Fructofuranosidase |
| Chemical | |
Reg. No./Substance:
|
0/Carbohydrates; 0/Enzymes, Immobilized; 0/Plant Lectins; 11028-71-0/Concanavalin A; EC 1.11.1.7/Peroxidase; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.26/beta-Fructofuranosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phosphatidylserine synthesis in phorbol ester treated glioma C6 cells.
Next Document: Plasma antioxidant enzymes and oxidized lipoproteins in hypercholesterolemic rabbits.