Document Detail


Covalent immobilization of ascorbate oxidase onto polycarbonate strip for l-ascorbic acid detection.
MedLine Citation:
PMID:  22704813     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Herein, a simple and rapid method is described for detection of l-ascorbic acid by ascorbate oxidase immobilized onto polycarbonate strip pre-activated by 1-fluoro-2-nitro-4-azidobenzene in photochemical reaction. Covalent attachment of ascorbate oxidase was confirmed by XPS studies. The immobilized-ascorbate oxidase shows higher pH, thermal and storage stability in comparison to free enzyme.
Authors:
Dileep Kumar Kannoujia; Saroj Kumar; Pradip Nahar
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-15
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  -     ISSN:  1347-4421     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Affiliation:
Institute of Genomics and Integrative Biology (CSIR), Mall Road, Delhi 110 007, India.
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