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Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM.
MedLine Citation:
PMID:  21629886     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A cross-metathesis reaction between an alkene terminated self-assembled monolayer (SAM) on glass/Si wafer and an alkene tethered Fischer carbene complex yielded a functionalized surface. Rapid aminolysis of the Fischer carbene moieties permit efficient anchoring of amine containing molecules on such a surface. Attachment of 1-pyrenemethylamine was thus monitored by ATR-IR spectroscopy and fluorescence microscopy. Similarly, BSA and lysozyme were individually grafted to such Fischer carbene modified surfaces using their pendant lysine residues. It has been demonstrated that the anchored lysozyme retains its bactericidal property.
Authors:
Piyali Dutta; Namrata Ray; Sarita Roy; Anjan Kr Dasgupta; Othman Bouloussa; Amitabha Sarkar
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-31
Journal Detail:
Title:  Organic & biomolecular chemistry     Volume:  -     ISSN:  1477-0539     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-6-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101154995     Medline TA:  Org Biomol Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Organic Chemistry, Indian Association for the Cultivation of Science, Kolkata-700032, India. ocas@iacs.res.in.
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