| Covalent bonding of protein to polyamine in the cyst coat of the protozoan Colpoda steinii. | |
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MedLine Citation:
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PMID: 6800792 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. High-voltage electrophoresis and chromatography before and after reaction with 5-dimethylaminonaphthalene-1-sulphonyl chloride have identified putrescine and spermidine in hydrolysates of cyst coat proteins from the protozoan Colpoda steinii. 2. Amounts present varied with putrescine up to 19.7 and spermidine up to 16.9 residues per 1000 amino acid residues. 3. The amines were not, in the main, removed by acid or alkaline extraction or by reprecipitation. They were present in hydrolysates of peptides isolated electrophoretically from acid-degraded coat protein. 4. Proteolysis of oxidised coat protein produced a soluble core polypeptide to which the major proportion of the amines were attached and which had a simple composition. It was composed almost entirely of glutamic acid or glutamine, glycine, serine and cysteic acid, these residues being present in the approximate ratio of 10:2:1:1. 5. When coat protein was treated with 5-dimethylaminonaphthalene-1-sulphonyl chloride and hydrolysed no fully substituted amines could be detected but putrescine with one group substituted and spermidine derivatives with one and two groups substituted were present. |
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Authors:
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J Tibbs |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 122 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1982 Mar |
Date Detail:
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Created Date: 1982-05-21 Completed Date: 1982-05-21 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 535-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Thin Layer Ciliophora / metabolism* Dansyl Compounds Hydrolysis Polyamines / metabolism* Protein Binding Putrescine / metabolism Spermidine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Dansyl Compounds; 0/Polyamines; 110-60-1/Putrescine; 124-20-9/Spermidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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