Document Detail


Covalent biofunctionalization of silicon nitride surfaces.
MedLine Citation:
PMID:  17455968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Covalently attached organic monolayers on etched silicon nitride (SixN4; x >/= 3) surfaces were prepared by reaction of SixN4-coated wafers with neat or solutions of 1-alkenes and 1-alkynes in refluxing mesitylene. The surface modification was monitored by measurement of the static water contact angle, XPS, IRRAS, AFM, and ToF-SIMS, and evidence for the formation of Si-C bonds is presented. The etching can be achieved by dilute HF solutions and yields both Si-H and N-H moieties. The resulting etched SixN4 surfaces are functionalized by terminal carboxylic acid groups in either of two ways: (a) via attachment of a 10-undecenoic acid 2,2,2-trifluoroethyl ester (trifluoro ethanol ester) and subsequent thermal acid hydrolysis; (b) through attachment of a photocleavable ester, and subsequent photochemical cleavage, as this would allow photopatterned functionalized SixN4. The carboxylic acids are successfully used for the attachment of oligopeptides (aspartame) and complete proteins using EDC/NHS chemistry. Finally, an amino-terminated organic monolayer can be formed by reaction of HF-treated SixN4 surfaces with a N-(omega-undecylenyl)phthalimide, which yields an amino-terminated surface upon deprotection with hydrazine.
Authors:
Ahmed Arafat; Marcel Giesbers; Michel Rosso; Ernst J R Sudhölter; Karin Schroën; Richard G White; Li Yang; Matthew R Linford; Han Zuilhof
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-25
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  23     ISSN:  0743-7463     ISO Abbreviation:  Langmuir     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-15     Completed Date:  2007-07-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6233-44     Citation Subset:  IM    
Affiliation:
Laboratory of Organic Chemistry, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Animals
Biosensing Techniques
Cattle
Coated Materials, Biocompatible / chemistry
Microscopy, Atomic Force
Oligopeptides / chemistry
Proteins / chemistry
Silicon Compounds / chemistry*
Spectrometry, Mass, Secondary Ion
Spectrum Analysis
Surface Properties
X-Rays
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Oligopeptides; 0/Proteins; 0/Silicon Compounds; 12033-89-5/silicon nitride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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