Document Detail

Covalent attachment of ribonucleic acids to proteins.
MedLine Citation:
PMID:  1280439     Owner:  NLM     Status:  MEDLINE    
As a prerequisite for the synthesis of affinity labels, we describe methods to couple histones to ribonucleic acids. For the synthesis of these covalent hybrid molecules, we used a population of histones H1, H2A, H2B, H3, and H4 from calf thymus and polyadenylic acid with an average chain length of up to 260-280 bases, representing the size of poly(A)-tails from mature mRNAs. Three methods were investigated. (a) Poly(A) containing an 8-N3-A residue was cross-linked to histones by ultraviolet irradiation. (b) The 3'-end of the polynucleotide was connected to a mononucleotide containing an aliphatic amino group, and the resulting poly(A)-derivative was coupled to histones via derivation with a bromoacetyl group. (c) The 3'-end of the polynucleotide was oxidized with sodium periodate and bound covalently to an amino group of the polypeptide. To demonstrate the RNA content of the hybrid molecule, the poly(A) was removed with RNase T2.
G Cremer; M Kalbas; H Fasold; D Prochnow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of protein chemistry     Volume:  11     ISSN:  0277-8033     ISO Abbreviation:  J. Protein Chem.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1993-01-04     Completed Date:  1993-01-04     Revised Date:  2008-08-19    
Medline Journal Info:
Nlm Unique ID:  8217321     Medline TA:  J Protein Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  553-60     Citation Subset:  IM    
Institut für Biochemie, Johann Wolfgang Goethe Universität, Frankfurt, Germany.
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MeSH Terms
Cross-Linking Reagents
Electrophoresis, Polyacrylamide Gel
Endoribonucleases / metabolism
Histones / metabolism*
Molecular Structure
Molecular Weight
Poly A / metabolism*
RNA / chemistry,  metabolism*
Thymus Gland / chemistry
Ultraviolet Rays
Uridine Monophosphate / analogs & derivatives,  metabolism
Reg. No./Substance:
0/Cross-Linking Reagents; 0/Histones; 144978-95-0/5-(3-(bromoacetamido)allyl)uridine-3'-monophosphate; 24937-83-5/Poly A; 58-97-9/Uridine Monophosphate; 63231-63-0/RNA; EC 3.1.-/Endoribonucleases; EC T(2)

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