Course introduction and selection of immunohistochemical staining panels: principles and importance of incorporating clinical information. The 5th annual retreat for applied immunohistochemistry and molecular pathology january 30th-february 2, 2011, coral gables, Florida.

Course introduction and selection of immunohistochemical staining panels: principles and importance of incorporating clinical information. The 5th annual retreat for applied immunohistochemistry and molecular pathology january 30th-february 2, 2011, coral gables, Florida.

MedLine Citation:	PMID: 22089485 Owner: NLM Status: In-Data-Review
Abstract/OtherAbstract:	: We are pleased to publish for the first time selected key summary articles of proceedings from the 5th Annual Retreat for Applied Immunohistochemistry and Molecular Pathology (AIMP). Although the utility and use of immunohistochemistry in diagnostic pathology has improved tremendously since its inception nearly 4 decades ago, issues continue in both technical and interpretive aspects of the method and in conforming with guidelines and regulatory requirements that are ever evolving for anatomic pathology laboratories. : The AIMP Retreat was born with this in mind, to provide up-to-date information for laboratory professionals, and a forum for sharing experience and expertise. The meeting, designed for both technologists and pathologists interested in and working in the fields of immunohistochemistry (IHC) and molecular pathology, began in 2007 under the directorship of one us, Hadi Yaziji, and with the support of The Society for Applied Immunohistochemistry. With a successful beginning in Duck Key, FL, the meeting has continued to grow in breadth of topics covered and diversity of faculty and attendees. The retreat has included a superb all day technical workshop detailing guiding principles of tissue fixation, processing and IHC technique, given by Dr Richard Cartun, Bryan Hewlett, and others. The remainder of the week has featured keynote speakers such as Drs Clive Taylor, Juan Rosai, Dr. Richard Cote and Frederick Becker. Presentations of IHC and molecular applications have covered most organ systems, including genitourinary, gastrointestinal, breast, lung, soft tissue, cutaneous, hematopoietic, and neuroendocrine systems, and the all-encompassing identification of infectious agents. Experts in these areas have included Drs Mahul Amin, Cyril Fisher, Allen Gown, Kevin Halling, Mehrdad Nadji, Gregory Lauwers, David Dabbs, Elizabeth Hammond, Stephen Hewitt, David Hicks, Todd Barry, Paul Swanson, William Travis, Alvin Martin, Michael Deavers, Savitri Krishnamurthy, Richard Cote, Richard Cartun, and Ronald DeLellis. Finally, other sessions have been devoted to video and digital image case presentations, ASCO-CAP guidelines and question and answer periods. Extensive interaction with the participants has become an integral and valued feature. : This year's featured speakers and topics included the keynote address on the state of molecular testing in colon cancer given by Dr Stanley Hamilton, new presentations on digital pathology and slide imaging, molecular assays in the diagnostic laboratory, standardization of pre-analytical test variables, and organ-based IHC applications in breast, lung, bladder, and prostate pathology. This issue of the Journal includes summaries of selected presentations and case studies. These are intended to highlight the key take home points, including the most up-to-date information, for each topic. : The intent is to provide the readers with an overview and useful quick reference of key content of the retreat and foster interest in meetings and symposia such as AIMP that are dedicated to IHC and related molecular applications in diagnostic pathology. We are very grateful to Dr Clive Taylor, the Journal Editor-in-Chief, the Editorial Board, and publisher, Lippincott, Williams and Wilkins, for giving us the opportunity to bring the proceedings to you and to the faculty for their generous contributions. : Hadi Yaziji, MD, AIMP Course Director Richard N. Eisen, MD, President, Society for Applied Immunohistochemistry ABSTRACT:: Among the core principles in the practice of immunohistochemistry is the use of carefully chosen marker panels. Choosing an appropriate panel of antibodies is predicated on a sound differential diagnosis that is based on detailed examination of hematoxylin and eosin-stained slides. The panel should contain antibodies designed to be immunoreactive in the most likely disease(s) in the differential as well as selected negative markers. In addition, the importance of detailed historical and clinical information in constructing the differential diagnosis and panel selection cannot be understated. Two cases from the Case Presentation sessions of the 5th Annual Retreat for Applied Immunohistochemistry and Molecular Pathology are summarized to illustrate these points. The first case is that of metastatic well-differentiated neuroendocrine tumor (carcinoid) tumor presenting as a breast mass. The second is that of a squamous cell carcinoma of the lung mimicking a tumor with admixed glandular differentiation by entrapment and disruption of bronchial glands. Application of a select immunohistochemistry panel in light of the differential diagnosis and importance of making a specific diagnosis are discussed.

Authors:	Richard N Eisen; Hadi Yaziji
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Publication Detail:	Type: Journal Article
Journal Detail:	Title: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry Volume: 19 ISSN: 1533-4058 ISO Abbreviation: Appl. Immunohistochem. Mol. Morphol. Publication Date: 2011 Dec
Date Detail:	Created Date: 2011-11-17 Completed Date: - Revised Date: -
Medline Journal Info:	Nlm Unique ID: 100888796 Medline TA: Appl Immunohistochem Mol Morphol Country: United States
Other Details:	Languages: eng Pagination: 485-90 Citation Subset: IM
Affiliation:	*Department of Pathology, Greenwich Hospital, Greenwich, CT †Vitro Molecular Laboratories, LLC, Miami, FL.
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