| Coupling between hydrodynamic forces and planar cell polarity orients mammalian motile cilia. | |
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MedLine Citation:
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PMID: 20305650 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In mammals, motile cilia cover many organs, such as fallopian tubes, respiratory tracts and brain ventricles. The development and function of these organs critically depend on efficient directional fluid flow ensured by the alignment of ciliary beating. To identify the mechanisms involved in this process, we analysed motile cilia of mouse brain ventricles, using biophysical and molecular approaches. Our results highlight an original orientation mechanism for ependymal cilia whereby basal bodies first dock apically with random orientations, and then reorient in a common direction through a coupling between hydrodynamic forces and the planar cell polarity (PCP) protein Vangl2, within a limited time-frame. This identifies a direct link between external hydrodynamic cues and intracellular PCP signalling. Our findings extend known PCP mechanisms by integrating hydrodynamic forces as long-range polarity signals, argue for a possible sensory role of ependymal cilia, and will be of interest for the study of fluid flow-mediated morphogenesis. |
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Authors:
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Boris Guirao; Alice Meunier; St?phane Mortaud; Andrea Aguilar; Jean-Marc Corsi; Laetitia Strehl; Yuki Hirota; Ang?lique Desoeuvre; Camille Boutin; Young-Goo Han; Zaman Mirzadeh; Harold Cremer; Mireille Montcouquiol; Kazunobu Sawamoto; Nathalie Spassky |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-21 |
Journal Detail:
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Title: Nature cell biology Volume: 12 ISSN: 1476-4679 ISO Abbreviation: Nat. Cell Biol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-02 Completed Date: 2010-04-26 Revised Date: 2010-05-05 |
Medline Journal Info:
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Nlm Unique ID: 100890575 Medline TA: Nat Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 341-50 Citation Subset: IM |
Affiliation:
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Institut de Biologie de l'Ecole Normale Sup?rieure (IBENS), Institut National de la Sant? et de la Recherche M?dicale U1024, Centre National de la Recherche Scientifique UMR8197, 75005 Paris, France. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Polarity* Cells, Cultured Cerebrospinal Fluid / metabolism Cilia / metabolism Ependyma / cytology*, embryology, metabolism Feedback, Physiological Humans Kinesin / metabolism Mechanotransduction, Cellular* Mice Mice, Transgenic Morphogenesis Motion Mutation Nerve Tissue Proteins / genetics, metabolism* Recombinant Fusion Proteins / metabolism Stress, Mechanical Time Factors Transfection Tumor Suppressor Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Kif3a protein, mouse; 0/Ltap protein, mouse; 0/Nerve Tissue Proteins; 0/Recombinant Fusion Proteins; 0/Tg737Rpw protein, mouse; 0/Tumor Suppressor Proteins; EC 3.6.1.-/Kinesin |
| Comments/Corrections | |
Comment In:
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Nat Cell Biol. 2010 Apr;12(4):314-5
[PMID:
20360747
]
Nat Rev Mol Cell Biol. 2010 May;11(5):313 [PMID: 20419890 ] |
Erratum In:
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Nat Cell Biol. 2010 May;12(5):520 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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