|Coupling of NF-protocadherin signaling to axon guidance by cue-induced translation.|
|PMID: 23292679 Owner: NLM Status: MEDLINE|
|Cell adhesion molecules and diffusible cues both regulate axon pathfinding, yet how these two modes of signaling interact is poorly understood. The homophilic cell adhesion molecule NF-protocadherin (NFPC) is expressed in the mid-dorsal optic tract neuroepithelium and in the axons of developing retinal ganglion cells (RGC) in Xenopus laevis. Here we report that targeted disruption of NFPC function in RGC axons or the optic tract neuroepithelium results in unexpectedly localized pathfinding defects at the caudal turn in the mid-optic tract. Semaphorin 3A (Sema3A), which lies adjacent to this turn, stimulates rapid, protein synthesis-dependent increases in growth cone NFPC and its cofactor, TAF1, in vitro. In vivo, growth cones exhibit marked increases in NFPC translation reporter activity in this mid-optic tract region that are attenuated by blocking neuropilin-1 function. Our results suggest that translation-linked coupling between regionally localized diffusible cues and cell adhesion can help axons navigate discrete segments of the pathway.|
|Louis C Leung; Vasja Urbančič; Marie-Laure Baudet; Asha Dwivedy; Timothy G Bayley; Aih Cheun Lee; William A Harris; Christine E Holt|
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|Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2013-01-06|
|Title: Nature neuroscience Volume: 16 ISSN: 1546-1726 ISO Abbreviation: Nat. Neurosci. Publication Date: 2013 Feb|
|Created Date: 2013-01-28 Completed Date: 2013-03-22 Revised Date: 2013-08-09|
Medline Journal Info:
|Nlm Unique ID: 9809671 Medline TA: Nat Neurosci Country: United States|
|Languages: eng Pagination: 166-73 Citation Subset: IM|
|Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.|
|APA/MLA Format Download EndNote Download BibTex|
Analysis of Variance
Anisomycin / pharmacology
Axons / physiology*
Cadherins / genetics, physiology*
Green Fluorescent Proteins / genetics, metabolism
Neuroepithelial Cells / physiology
Organ Culture Techniques
Protein Biosynthesis* / drug effects, physiology
Protein Synthesis Inhibitors / pharmacology
Retina / cytology
Retinal Ganglion Cells / cytology*
Semaphorin-3A / pharmacology
Signal Transduction / drug effects, physiology*
Transcription Factors / metabolism
Visual Pathways / physiology*
Xenopus Proteins / genetics, physiology*
|085314//Wellcome Trust; 085314/Z/08/Z//Wellcome Trust; //Medical Research Council|
|0/Cadherins; 0/NFPC protein, Xenopus; 0/Protein Synthesis Inhibitors; 0/Semaphorin-3A; 0/Transcription Factors; 0/Xenopus Proteins; 147336-22-9/Green Fluorescent Proteins; 22862-76-6/Anisomycin|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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