| Coupled-enzyme system for the determination of lipase activity. | |
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MedLine Citation:
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PMID: 15483386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new method for lipase activity determination is described, in which liberation of fatty acids by lipase-catalyzed hydrolysis is coupled to lipoxygenation. The second reaction forms hydroperoxy-fatty acids containing a conjugated double bond that are detected at 234 nm. The method is sensitive, cheap and easy to use when compared to a titration method. |
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Authors:
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Ines Ben Rejeb; Jeannette Ben Hamida; Mohamed Gargouri |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Biotechnology letters Volume: 26 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-10-14 Completed Date: 2004-12-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1273-6 Citation Subset: IM |
Affiliation:
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Department of Biological & Chemical Engineering, National Institute of Applied Science and Technology (INSAT), Tunis, Tunisia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Catalysis Coenzymes / analysis, chemistry Enzyme Activation Fatty Acids / chemistry* Hydrolysis Lipase / analysis*, chemistry* Oxygen / chemistry* Spectrophotometry / methods* Triglycerides / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Coenzymes; 0/Fatty Acids; 0/Triglycerides; 537-40-6/trilinolein; 7782-44-7/Oxygen; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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