Document Detail

Coupled-enzyme system for the determination of lipase activity.
MedLine Citation:
PMID:  15483386     Owner:  NLM     Status:  MEDLINE    
A new method for lipase activity determination is described, in which liberation of fatty acids by lipase-catalyzed hydrolysis is coupled to lipoxygenation. The second reaction forms hydroperoxy-fatty acids containing a conjugated double bond that are detected at 234 nm. The method is sensitive, cheap and easy to use when compared to a titration method.
Ines Ben Rejeb; Jeannette Ben Hamida; Mohamed Gargouri
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Biotechnology letters     Volume:  26     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-10-14     Completed Date:  2004-12-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1273-6     Citation Subset:  IM    
Department of Biological & Chemical Engineering, National Institute of Applied Science and Technology (INSAT), Tunis, Tunisia.
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MeSH Terms
Coenzymes / analysis,  chemistry
Enzyme Activation
Fatty Acids / chemistry*
Lipase / analysis*,  chemistry*
Oxygen / chemistry*
Spectrophotometry / methods*
Triglycerides / chemistry*
Reg. No./Substance:
0/Coenzymes; 0/Fatty Acids; 0/Triglycerides; 537-40-6/trilinolein; 7782-44-7/Oxygen; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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