Document Detail


Coupled enzymatic assay for estimation of branched-chain L-amino acid aminotransferase activity with 2-Oxo acid substrates.
MedLine Citation:
PMID:  8660588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A convenient continuous spectrophotometric assay for estimation of branched-chain L-amino acid aminotransferase activity was established: Branched-chain 2-oxo acid-dependent transamination of L-glutamate was coupled-via 2-oxoglutarate-to L-aspartate aminotransferase plus L-malate dehydrogenase or to L-alanine aminotransferase plus L-lactate dehydrogenase as indicator systems. The rate of transamination can be monitored specifically by measuring the decrease in NADH absorbance at 334 nm over time. The method was applied, e.g., for evaluation of some kinetic properties of the rat heart (iso)enzyme.
Authors:
P Schadewaldt; F Adelmeyer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  238     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-09-04     Completed Date:  1996-09-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  65-71     Citation Subset:  IM    
Affiliation:
Institut für Physiologische Chemie II, Heinrich-Heine-Universität Düsseldorf, Auf'm Hennekamp 65, Düsseldorf, D-40225, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell-Free System / enzymology
Cells, Cultured
Endothelium, Vascular / cytology,  enzymology
Isoenzymes / analysis
Keto Acids / chemistry*
Linear Models
Mitochondria, Heart / enzymology*
Rats
Substrate Specificity
Temperature
Transaminases / analysis*
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Keto Acids; 1460-34-0/alpha-keto-beta-methylvaleric acid; 759-05-7/alpha-ketoisovalerate; 816-66-0/alpha-ketoisocaproic acid; EC 2.6.1.-/Transaminases; EC 2.6.1.42/branched-chain-amino-acid transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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