| Coupled enzymatic assay for estimation of branched-chain L-amino acid aminotransferase activity with 2-Oxo acid substrates. | |
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MedLine Citation:
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PMID: 8660588 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A convenient continuous spectrophotometric assay for estimation of branched-chain L-amino acid aminotransferase activity was established: Branched-chain 2-oxo acid-dependent transamination of L-glutamate was coupled-via 2-oxoglutarate-to L-aspartate aminotransferase plus L-malate dehydrogenase or to L-alanine aminotransferase plus L-lactate dehydrogenase as indicator systems. The rate of transamination can be monitored specifically by measuring the decrease in NADH absorbance at 334 nm over time. The method was applied, e.g., for evaluation of some kinetic properties of the rat heart (iso)enzyme. |
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Authors:
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P Schadewaldt; F Adelmeyer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 238 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-09-04 Completed Date: 1996-09-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 65-71 Citation Subset: IM |
Affiliation:
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Institut für Physiologische Chemie II, Heinrich-Heine-Universität Düsseldorf, Auf'm Hennekamp 65, Düsseldorf, D-40225, Federal Republic of Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell-Free System / enzymology Cells, Cultured Endothelium, Vascular / cytology, enzymology Isoenzymes / analysis Keto Acids / chemistry* Linear Models Mitochondria, Heart / enzymology* Rats Substrate Specificity Temperature Transaminases / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 0/Keto Acids; 1460-34-0/alpha-keto-beta-methylvaleric acid; 759-05-7/alpha-ketoisovalerate; 816-66-0/alpha-ketoisocaproic acid; EC 2.6.1.-/Transaminases; EC 2.6.1.42/branched-chain-amino-acid transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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