Document Detail

Counting leukocytes from whole blood using a lab-on-a-chip Coulter counter.
MedLine Citation:
PMID:  23367364     Owner:  NLM     Status:  MEDLINE    
A microfluidic lab-on-a-chip Coulter counter was demonstrated to count micro particles and leukocytes from whole blood. Instead of electroplated or deposited metal electrodes, off-the-shelf gold pins were used as electrodes to simplify fabrication process, reduce cost, enhance device durability, and above all, achieve superior uniformity in E-field distribution for improved signal quality. A custom-designed, low-cost demodulation circuit was developed to detect the AC impedance signals of the particles and cells passing the detection area defined by the microfluidic channels. A mixture of polystyrene beads with three different sizes was used to characterize the device. The results showed high throughput at 2000 particles/s and clear separation among different sizes of beads with coefficients of variation (CV) of 13.53%, 10.35% and 5.67% for 7.66 µm, 10.5 µm and 14.7 µmbeads, respectively. To demonstrate the potential for a point-of-care or self-administered device for cancer patients going through chemotherapy, we have used the lab-on-a-chip device to count leukocytes from whole blood, generating encouraging preliminary results comparable to the results from a commercial flow cytometer.
Zhe Mei; Sung Hwan Cho; Arthur Zhang; Jie Dai; Tsung-Feng Wu; Yu-Hwa Lo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference     Volume:  2012     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2012  
Date Detail:
Created Date:  2013-01-31     Completed Date:  2013-07-23     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6277-80     Citation Subset:  IM    
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MeSH Terms
Cost Control
Lab-On-A-Chip Devices*
Leukocyte Count*
Neoplasms / blood
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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