Document Detail


Counting bacteria using functionalized gold nanoparticles (AuNPs) as the light-scattering reporter.
MedLine Citation:
PMID:  23035847     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A simple and rapid bacterial counting method was developed based on dark-field light-scattering imaging of bacteria and gold nanoparticle (AuNP) reporter simultaneously. Commercially available DH5α E. coli strain was used as the model bacterium to demonstrate the feasibility of the proposed method. With antibody-conjugated AuNPs, the simple sample treatment and target E. coli strain recognition can be finished within 15~30 min, with a detection limit of 2~6×10(4) colony forming unit per milliliter (CFU/mL). By using 90-nm AuNPs as the light-scattering signal reporter, the bacterial recognition and counting can be easily performed with low-cost instrumentation such as an entry-level dark-field microscope setup and a common tungsten lamp as the light source. An automatic image analysis algorithm was also developed to facilitate robust and fast bacterial counting. The preliminary results of water, milk and fruit juice sample analysis showed that this simple, fast and cost-effective method can be easily adopted for routine bacterial detection.
Authors:
Xiao Xu; Yang Chen; Hejia Wei; Bin Xia; Feng Liu; Na Li
Related Documents :
8218907 - Actinic light density dependence of the bacteriorhodopsin protocycle.
17360007 - On a pulsating brownian motor and its characterization.
16172377 - Effect of temperature on the working stroke of muscle myosin.
23072047 - Effect of inclination and position of new swimming starting block's back plate on track...
21056097 - A lateralized auditory evoked potential elicited when auditory objects are defined by s...
3978427 - Bicuculline blocks circadian phase delays but not advances.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inter-domain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA syntheta...
Next Document:  The significance of early accumulation of nanomolar concentrations of NO as an inducer of denitrific...