Document Detail

Counting bacteria using functionalized gold nanoparticles (AuNPs) as the light-scattering reporter.
MedLine Citation:
PMID:  23035847     Owner:  NLM     Status:  Publisher    
A simple and rapid bacterial counting method was developed based on dark-field light-scattering imaging of bacteria and gold nanoparticle (AuNP) reporter simultaneously. Commercially available DH5α E. coli strain was used as the model bacterium to demonstrate the feasibility of the proposed method. With antibody-conjugated AuNPs, the simple sample treatment and target E. coli strain recognition can be finished within 15~30 min, with a detection limit of 2~6×10(4) colony forming unit per milliliter (CFU/mL). By using 90-nm AuNPs as the light-scattering signal reporter, the bacterial recognition and counting can be easily performed with low-cost instrumentation such as an entry-level dark-field microscope setup and a common tungsten lamp as the light source. An automatic image analysis algorithm was also developed to facilitate robust and fast bacterial counting. The preliminary results of water, milk and fruit juice sample analysis showed that this simple, fast and cost-effective method can be easily adopted for routine bacterial detection.
Xiao Xu; Yang Chen; Hejia Wei; Bin Xia; Feng Liu; Na Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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