Document Detail


Counting ions around DNA with anomalous small-angle X-ray scattering.
MedLine Citation:
PMID:  21047071     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The majority of charge-compensating ions around nucleic acids form a diffuse counterion "cloud" that is not amenable to investigation by traditional methods that rely on rigid structural interactions. Although various techniques have been employed to characterize the ion atmosphere around nucleic acids, only anomalous small-angle X-ray scattering (ASAXS) provides information about the spatial distribution of ions. Here we present an experimentally straightforward extension of ASAXS that can be used to count the number of ions around nucleic acids.
Authors:
Suzette A Pabit; Steve P Meisburger; Li Li; Joshua M Blose; Christopher D Jones; Lois Pollack
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-11-03
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  132     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-18     Completed Date:  2011-03-08     Revised Date:  2011-12-21    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  16334-6     Citation Subset:  IM    
Affiliation:
School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, United States.
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MeSH Terms
Descriptor/Qualifier:
DNA / chemistry*
Ions
Scattering, Small Angle*
X-Rays
Grant Support
ID/Acronym/Agency:
GM-076121-04S1/GM/NIGMS NIH HHS; GM-085062/GM/NIGMS NIH HHS; GM-088645/GM/NIGMS NIH HHS; R01 GM076121-04S1/GM/NIGMS NIH HHS; R01 GM085062-01A2/GM/NIGMS NIH HHS; R01 GM085062-03/GM/NIGMS NIH HHS; R01 GM088645-01/GM/NIGMS NIH HHS; R01 GM088645-03/GM/NIGMS NIH HHS; T32 GM-008267/GM/NIGMS NIH HHS; T32 GM008267-18/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Ions; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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