Document Detail


Counterflow dielectrophoresis for trypanosome enrichment and detection in blood.
MedLine Citation:
PMID:  23105971     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human African trypanosomiasis or sleeping sickness is a deadly disease endemic in sub-Saharan Africa, caused by single-celled protozoan parasites. Although it has been targeted for elimination by 2020, this will only be realized if diagnosis can be improved to enable identification and treatment of afflicted patients. Existing techniques of detection are restricted by their limited field-applicability, sensitivity and capacity for automation. Microfluidic-based technologies offer the potential for highly sensitive automated devices that could achieve detection at the lowest levels of parasitemia and consequently help in the elimination programme. In this work we implement an electrokinetic technique for the separation of trypanosomes from both mouse and human blood. This technique utilises differences in polarisability between the blood cells and trypanosomes to achieve separation through opposed bi-directional movement (cell counterflow). We combine this enrichment technique with an automated image analysis detection algorithm, negating the need for a human operator.
Authors:
Anoop Menachery; Clemens Kremer; Pui E Wong; Allan Carlsson; Steven L Neale; Michael P Barrett; Jonathan M Cooper
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-26
Journal Detail:
Title:  Scientific reports     Volume:  2     ISSN:  2045-2322     ISO Abbreviation:  Sci Rep     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-29     Completed Date:  2013-03-25     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101563288     Medline TA:  Sci Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  775     Citation Subset:  IM    
Affiliation:
Department of Electronics and Electrical Engineering, The Bioelectronics Research Centre, College of Science and Engineering, Rankine Building, University of Glasgow, Glasgow G12 8LT, Scotland, UK.
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Electrophoresis / methods
Humans
Mice
Parasitemia
Trypanosoma / isolation & purification*
Trypanosomiasis, African / blood,  diagnosis*,  parasitology
Grant Support
ID/Acronym/Agency:
085349//Wellcome Trust
Comments/Corrections

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