| Counterflow dielectrophoresis for trypanosome enrichment and detection in blood. | |
| | |
MedLine Citation:
|
PMID: 23105971 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Human African trypanosomiasis or sleeping sickness is a deadly disease endemic in sub-Saharan Africa, caused by single-celled protozoan parasites. Although it has been targeted for elimination by 2020, this will only be realized if diagnosis can be improved to enable identification and treatment of afflicted patients. Existing techniques of detection are restricted by their limited field-applicability, sensitivity and capacity for automation. Microfluidic-based technologies offer the potential for highly sensitive automated devices that could achieve detection at the lowest levels of parasitemia and consequently help in the elimination programme. In this work we implement an electrokinetic technique for the separation of trypanosomes from both mouse and human blood. This technique utilises differences in polarisability between the blood cells and trypanosomes to achieve separation through opposed bi-directional movement (cell counterflow). We combine this enrichment technique with an automated image analysis detection algorithm, negating the need for a human operator. |
| | |
Authors:
|
Anoop Menachery; Clemens Kremer; Pui E Wong; Allan Carlsson; Steven L Neale; Michael P Barrett; Jonathan M Cooper |
Publication Detail:
|
Type: Journal Article Date: 2012-10-26 |
Journal Detail:
|
Title: Scientific reports Volume: 2 ISSN: 2045-2322 ISO Abbreviation: Sci Rep Publication Date: 2012 |
Date Detail:
|
Created Date: 2012-10-29 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101563288 Medline TA: Sci Rep Country: England |
Other Details:
|
Languages: eng Pagination: 775 Citation Subset: IM |
Affiliation:
|
Department of Electronics and Electrical Engineering, The Bioelectronics Research Centre, College of Science and Engineering, Rankine Building, University of Glasgow , Glasgow, G12 8LT, Scotland, U.K. ; Both authors have contributed equally to this study. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transparent, flexible supercapacitors from nano-engineered carbon films.
Next Document: Simultaneous detection of ricin and abrin DNA by real-time PCR (qPCR).