| Counter-current chromatographic separation of nucleic acid constituents with a hydrophilic solvent system. | |
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MedLine Citation:
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PMID: 20362294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acid constituents such as nucleobases, nucleosides and nucleotides were separated by counter-current chromatography using type J coil planet centrifuge. The separation was performed with a hydrophilic solvent system composed of 1-propanol/800 mM potassium phosphate buffer (pH 7.4) (1:1, v/v) by eluting the lower aqueous phase at a flow-rate of 0.5 ml/min. Eight selected nucleic acid constituents (4.0 mg, 0.5 mg of each), uridine monophosphate (UMP), adenosine monophosphate (AMP), deoxyadenosine monophosphate (dAMP), uridine, urasile, deoxy uridine, adenosine and adenine were well resolved within 160 min. |
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Authors:
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Yoichi Shibusawa; Atsushi Shoji; Chihiro Suzuka; Akio Yanagida; Yoichiro Ito |
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Publication Detail:
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Type: Journal Article Date: 2010-03-16 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1217 ISSN: 1873-3778 ISO Abbreviation: J Chromatogr A Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-26 Completed Date: 2010-07-19 Revised Date: 2012-03-08 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 3457-60 Citation Subset: IM |
Copyright Information:
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2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Division of Pharmaceutical and Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. sibusawa@toyaku.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Countercurrent Distribution
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methods* Hydrophobic and Hydrophilic Interactions Nucleic Acids / chemistry, isolation & purification* Solvents / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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Z99 HL999999/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 0/Solvents |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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