Document Detail

Cotton Transformation via Pollen Tube Pathway.
MedLine Citation:
PMID:  23143484     Owner:  NLM     Status:  In-Data-Review    
Although many gene transfer methods have been employed for successfully obtaining transgenic cotton, the major constraint in cotton improvement is the limitation of genotype because the majority of transgenic methods require plant regeneration from a single transformed cell which is limited by cotton tissue culture. Comparing with other plant species, it is difficult to induce plant regeneration from cotton; currently, only a limited number of cotton cultivars can be cultured for obtaining regenerated plants. Thus, development of a simple and genotype-independent genetic transformation method is particularly important for cotton community. In this chapter, we present a simple, cost-efficient, and genotype-independent cotton transformation method-pollen tube pathway-mediated transformation. This method uses pollen tube pathway to deliver transgene into cotton embryo sacs and then insert foreign genes into cotton genome. There are three major steps for pollen tube pathway-mediated genetic transformation, which include injection of -foreign genes into pollen tube, integration of foreign genes into plant genome, and selection of transgenic plants.
Min Wang; Baohong Zhang; Qinglian Wang
Related Documents :
24301544 - Digestion and absorption ofeucalyptus essential oils in greater glider (petauroide svol...
24767494 - Biomass fly ashes as low-cost chemical agents for pb removal from synthetic and industr...
16781804 - Can we predict uranium bioavailability based on soil parameters? part 2: soil solution ...
18995934 - Radon-222 exhalation from open ground on and around a uranium mine in the wet-dry tropics.
25469984 - The biogeochemical role of baleen whales and krill in southern ocean nutrient cycling.
2205214 - Elicitor recognition and signal transduction in plant defense gene activation.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  958     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  71-7     Citation Subset:  IM    
Beijing Key Laboratory of Plant Resources Research and Development, Department of Biotechnology, School of Science, Beijing Technology and Business University, Haidian District, Beijing, People's Republic of China,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Biolistic transformation of cotton embryogenic cell suspension cultures.
Next Document:  Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.).