| Cotinine in Human Placenta Predicts Induction of Gene Expression in Fetal Tissues. | |
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MedLine Citation:
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PMID: 23209192 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Maternal cigarette smoking during pregnancy is associated with increased risk of perinatal morbidity and mortality. However, the mechanisms underlying adverse birth outcomes following prenatal exposure to cigarette smoke remain unknown due, in part, to the absence or unreliability of information regarding maternal cigarette smoke exposure during pregnancy. Our goal was to determine if placental cotinine could be a reliable biomarker of fetal cigarette smoke exposure during pregnancy. Cotinine levels were determined in placentas from 47 women who reported smoking during pregnancy and 10 from women who denied cigarette smoke exposure. Cotinine levels were significantly higher in placentas from women reporting cigarette smoking (median=27.2 ng/g) versus women who reported no smoke exposure (2.3 ng/g, P<0.001). Receiver operating characteristic curve analysis identified an optimal cut point of 7.5 ng/g (sensitivity=78.7%, specificity=100%) to classify placenta samples from mothers who smoked from those who did not. Among 415 placentas for which maternal cigarette smoking status was unavailable, 167 had cotinine levels >7.5 ng/g and would be considered positive for cigarette smoke exposure. Data from quantitative RT-PCR analyses demonstrated that in utero cigarette smoke exposure predicted by cotinine in placenta is associated with changes in the expression of xenobiotic-metabolizing enzymes in fetal tissues. CYP1A1 mRNA in fetal lung and liver and CYP1B1 in fetal lung were significantly induced when cotinine was detected in placenta. These findings indicate that cotinine in placenta is a reliable biomarker for fetal exposure and response to maternal cigarette smoking during pregnancy. |
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Authors:
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Carrie A Vyhlidal; Amanda K Riffel; Kathleen J Haley; Sunita Sharma; Hongying Dai; Kelan G Tantisira; Scott T Weiss; J Steven Leeder |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-3 |
Journal Detail:
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Title: Drug metabolism and disposition: the biological fate of chemicals Volume: - ISSN: 1521-009X ISO Abbreviation: Drug Metab. Dispos. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421550 Medline TA: Drug Metab Dispos Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1 Children's Mercy Hospital and Clinics; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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