| Cotinine exposure increases Fallopian tube PROKR1 expression via nicotinic AChRalpha-7: a potential mechanism explaining the link between smoking and tubal ectopic pregnancy. | |
| | |
MedLine Citation:
|
PMID: 20864676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Tubal ectopic pregnancy (EP) is the most common cause of maternal mortality in the first trimester of pregnancy; however, its etiology is uncertain. In EP, embryo retention within the Fallopian tube (FT) is thought to be due to impaired smooth muscle contractility (SMC) and alterations in the tubal microenvironment. Smoking is a major risk factor for EP. FTs from women with EP exhibit altered prokineticin receptor-1 (PROKR1) expression, the receptor for prokineticins (PROK). PROK1 is angiogenic, regulates SMC, and is involved in intrauterine implantation. We hypothesized that smoking predisposes women to EP by altering tubal PROKR1 expression. Sera/FT were collected at hysterectomy (n=21). Serum levels of the smoking metabolite, cotinine, were measured by enzyme-linked immunosorbent assay. FTs were analyzed by q-RT-PCR, immunohistochemistry, and Western blotting for expression of PROKR1 and the predicted cotinine receptor, nicotinic acetylcholine receptor α-7 (AChRα-7). FT explants (n=4) and oviductal epithelial cells (cell line OE-E6/E7) were treated with cotinine and an nAChRα-7 antagonist. PROKR1 transcription was higher in FTs from smokers (P<0.01). nAChRα-7 expression was demonstrated in FT epithelium. Cotinine treatment of FT explants and OE-E6/E7 cells increased PROKR1 expression (P<0.05), which was negated by cotreatment with nAChRα-7 antagonist. Smoking targets human FTs via nAChRα-7 to increase tubal PROKR1, leading to alterations in the tubal microenvironment that could predispose to EP. |
| | |
Authors:
|
Julie L V Shaw; Elizabeth Oliver; Kai-Fai Lee; Gary Entrican; Henry N Jabbour; Hilary O D Critchley; Andrew W Horne |
Related Documents
:
|
281276 - Anatomic relationshiphs between the human levator and tensor veli palatini and the eust... 10710736 - Pregnancy rates after laparoscopic treatment. differences related to tubal status and p... 3709826 - Peritoneal endometriosis in women requesting reversal of sterilization. 1267236 - Sequential sterile autolysis in the ovine fetus: macroscopic changes. 12562946 - The dangerous road of catch-up growth. 10846336 - Misoprostol alone--a new method of medical abortion? |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-23 |
Journal Detail:
|
Title: The American journal of pathology Volume: 177 ISSN: 1525-2191 ISO Abbreviation: Am. J. Pathol. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-11-04 Completed Date: 2011-03-21 Revised Date: 2013-05-27 |
Medline Journal Info:
|
Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: United States |
Other Details:
|
Languages: eng Pagination: 2509-15 Citation Subset: AIM; IM |
Affiliation:
|
Centre for Reproductive Biology, University of Edinburgh, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Animals Bungarotoxins / pharmacology Cell Line Cotinine / blood, pharmacology* Fallopian Tubes / anatomy & histology, drug effects*, metabolism* Female Humans Indicators and Reagents Middle Aged Pregnancy Pregnancy, Ectopic / etiology* Receptors, G-Protein-Coupled / genetics, metabolism* Receptors, Nicotinic / metabolism* Smoking / adverse effects* Tissue Culture Techniques Vascular Endothelial Growth Factor, Endocrine-Gland-Derived / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
G0802808//Medical Research Council; G0802808(90914)//Medical Research Council; U.1276.00.004.00002.02//Medical Research Council |
| Chemical | |
Reg. No./Substance:
|
0/Bungarotoxins; 0/Indicators and Reagents; 0/PROKR1 protein, human; 0/Receptors, G-Protein-Coupled; 0/Receptors, Nicotinic; 0/Vascular Endothelial Growth Factor, Endocrine-Gland-Derived; 0/alpha7 nicotinic acetylcholine receptor; 486-56-6/Cotinine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neuroserpin protects neurons from ischemia-induced plasmin-mediated cell death independently of tiss...
Next Document: Toll-like receptor (TLR) 2 and TLR9 expressed in trigeminal ganglia are critical to viral control du...