Document Detail

Cost-effective recovery and purification of polyhydroxyalkanoates by selective dissolution of cell mass.
MedLine Citation:
PMID:  16599575     Owner:  NLM     Status:  MEDLINE    
Highly efficient separation and purification of polyhydroxyalkanoates (PHAs) from PHA-containing cell mass is essential to production of the bioplastics from renewable resources in a cost-effective, environmentally friendly way. Based on selective dissolution of non-PHA cell mass (NPCM) by protons in aqueous solution and crystallization kinetics of PHA biopolymers, a simple process is developed and demonstrated to recover PHAs from cell mass to high purity (>97 wt %) with high yield (>95 wt %). The average molecular weight of biopolyesters is controlled, which follows an exponential function of process severity, a combined factor of processing conditions. Compared with conventional chemical treatment such as sequential surfactant and hypochlorite treatment, this new technology substantially reduces the chemical cost for PHA recovery and purification from PHA-containing cell mass.
Jian Yu; Lilian X L Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  22     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2006 Mar-Apr
Date Detail:
Created Date:  2006-04-07     Completed Date:  2006-06-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  547-53     Citation Subset:  IM    
Hawaii Natural Energy Institute, University of Hawaii, Honolulu, 96822, USA.
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MeSH Terms
Alkenes / chemistry*,  isolation & purification*,  metabolism
Biopolymers / biosynthesis,  chemistry,  isolation & purification
Cupriavidus necator / chemistry,  metabolism
Hydroxybutyrates / chemistry
Molecular Weight
Polyesters / chemistry
Proteins / chemistry
Solutions / economics
Spectrophotometry, Infrared
Surface-Active Agents / economics
Reg. No./Substance:
0/Alkalies; 0/Alkenes; 0/Biopolymers; 0/Hydroxybutyrates; 0/Polyesters; 0/Proteins; 0/Protons; 0/Solutions; 0/Surface-Active Agents; 26063-00-3/poly-beta-hydroxybutyrate

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