Document Detail

Cosmid clones from microdissected human chromosomal region 15q11-q13.
MedLine Citation:
PMID:  8260719     Owner:  NLM     Status:  MEDLINE    
A human chromosomal region, 15q11-q13, was microdissected, its DNA was amplified with the primer-linker PCR method, and the PCR products were cloned into a plasmid vector to construct a microclone library. Of 193 microclones analyzed with Southern blot hybridization on hybrid cell panels, 26 (13.5%) were either single-copy (unique) or low-repetitive fragments. By screening of a cosmid library of human genomic DNA using the 26 microclones as probes, 47 positive cosmids were obtained and underwent regional mapping with chromosome fluorescence in situ hybridization (FISH). Sixteen cosmids gave FISH signals at 15p-cen, 5 at 15q11-q13, 6 at 15q22-q26, 3 at other chromosomes, and 17 no signal. These 27 cosmids mapped to chromosome 15 are useful additions to the inventory of DNA markers of this chromosome including the much interested Prader-Willi/Angelman syndrome region.
T Tohma; T Tamura; T Ohta; H Soejima; T Kubota; Y Jinno; K Tsukamoto; Y Nakamura; K Naritomi; N Niikawa
Related Documents :
16133449 - A population-based ld map of the human chromosome 6p.
8529869 - New tools for integrated genetic and physical analyses of the escherichia coli chromosome.
11447249 - A cryptic rry(i) microsatellite from atlantic salmon (salmo salar): characterization an...
10593079 - First-generation physical map of the culicoides variipennis (diptera: ceratopogonidae) ...
8416889 - Genomic restriction map of the extremely thermophilic bacterium thermus thermophilus hb8.
8661029 - A human chromosome 22 fosmid resource: mapping and analysis of 96 clones.
7642709 - The checkpoint delaying anaphase in response to chromosome monoorientation is mediated ...
8595049 - S-related protein can be recombined with self-compatibility in interspecific derivative...
1628819 - Mapping and characterization of a 'speciation gene' in drosophila.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Japanese journal of human genetics     Volume:  38     ISSN:  0916-8478     ISO Abbreviation:  Jpn. J. Hum. Genet.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1994-01-27     Completed Date:  1994-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9213239     Medline TA:  Jpn J Hum Genet     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  267-75     Citation Subset:  IM    
Department of Pediatrics, University of the Ryukyus School of Medicine, Okinawa, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Base Sequence
Chromosomes, Human, Pair 15*
Cloning, Molecular
Cosmids / genetics*
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Polymerase Chain Reaction
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Detection of monosomy 7 by fluorescence in situ hybridization in acute nonlymphocytic leukemia and m...
Next Document:  Sexing of in vitro-fertilized preimplantation mouse embryos by the PCR method.