Document Detail


Cosmid clones from microdissected human chromosomal region 15q11-q13.
MedLine Citation:
PMID:  8260719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human chromosomal region, 15q11-q13, was microdissected, its DNA was amplified with the primer-linker PCR method, and the PCR products were cloned into a plasmid vector to construct a microclone library. Of 193 microclones analyzed with Southern blot hybridization on hybrid cell panels, 26 (13.5%) were either single-copy (unique) or low-repetitive fragments. By screening of a cosmid library of human genomic DNA using the 26 microclones as probes, 47 positive cosmids were obtained and underwent regional mapping with chromosome fluorescence in situ hybridization (FISH). Sixteen cosmids gave FISH signals at 15p-cen, 5 at 15q11-q13, 6 at 15q22-q26, 3 at other chromosomes, and 17 no signal. These 27 cosmids mapped to chromosome 15 are useful additions to the inventory of DNA markers of this chromosome including the much interested Prader-Willi/Angelman syndrome region.
Authors:
T Tohma; T Tamura; T Ohta; H Soejima; T Kubota; Y Jinno; K Tsukamoto; Y Nakamura; K Naritomi; N Niikawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Japanese journal of human genetics     Volume:  38     ISSN:  0916-8478     ISO Abbreviation:  Jpn. J. Hum. Genet.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1994-01-27     Completed Date:  1994-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9213239     Medline TA:  Jpn J Hum Genet     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  267-75     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, University of the Ryukyus School of Medicine, Okinawa, Japan.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosomes, Human, Pair 15*
Cloning, Molecular
Cosmids / genetics*
DNA*
Humans
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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