| Cortisol is a suppressor of apoptosis in bovine corpus luteum. | |
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MedLine Citation:
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PMID: 18218610 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucocorticoid (GC) acts as a modulator of physiological functions in several organs. In the present study, we examined whether GC suppresses luteolysis in bovine corpus luteum (CL). Cortisol (an active GC) reduced the mRNA expression of caspase 8 (CASP8) and caspase 3 (CASP3) and reduced the enzymatic activity of CASP3 and cell death induced by tumor necrosis factor (TNF) and interferon gamma (IFNG) in cultured bovine luteal cells. mRNAs and proteins of GC receptor (NR3C1), 11beta-hydroxysteroid dehydrogenase type 1 (HSD11B1), and HSD11B2 were expressed in CL throughout the estrous cycle. Moreover, the protein expression and the enzymatic activity of HSD11B1 were high at the early and the midluteal stages compared to the regressed luteal stage. These results suggest that cortisol suppresses TNF-IFNG-induced apoptosis in vitro by reducing apoptosis signals via CASP8 and CASP3 in bovine CL and that the local increase in cortisol production resulting from increased HSD11B1 at the early and midluteal stages helps to maintain CL function by suppressing apoptosis of luteal cells. |
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Authors:
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Junichi Komiyama; Ryo Nishimura; Hwa-Yong Lee; Ryosuke Sakumoto; Masafumi Tetsuka; Tomas J Acosta; Dariusz J Skarzynski; Kiyoshi Okuda |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-23 |
Journal Detail:
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Title: Biology of reproduction Volume: 78 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-23 Completed Date: 2008-07-17 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 888-95 Citation Subset: IM |
Affiliation:
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Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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11-beta-Hydroxysteroid Dehydrogenase Type 1
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metabolism 11-beta-Hydroxysteroid Dehydrogenase Type 2 / metabolism Animals Apoptosis / drug effects, physiology* Caspase 3 / metabolism Caspase 8 / metabolism Cattle Cells, Cultured Corpus Luteum / cytology*, drug effects, metabolism Fas Ligand Protein / metabolism Female Hydrocortisone / pharmacology, physiology* Interferon-gamma / pharmacology Luteal Cells / cytology*, drug effects, metabolism RNA, Messenger / metabolism Receptors, Glucocorticoid / metabolism Signal Transduction / physiology Tumor Necrosis Factor-alpha / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Fas Ligand Protein; 0/RNA, Messenger; 0/Receptors, Glucocorticoid; 0/Tumor Necrosis Factor-alpha; 50-23-7/Hydrocortisone; 82115-62-6/Interferon-gamma; EC 1.1.1.146/11-beta-Hydroxysteroid Dehydrogenase Type 1; EC 1.1.1.146/11-beta-Hydroxysteroid Dehydrogenase Type 2; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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