Document Detail

Cortisol is not the primary mediator for augmented CXCR4 expression on natural killer cells after acute exercise.
MedLine Citation:
PMID:  24947029     Owner:  NLM     Status:  Publisher    
CXC-chemokine receptor 4 (CXCR4) and its ligand, stromal-derived factor 1α (SDF-1αalso known as CXCL12), are crucial for the redistribution of immune cells after acute exercise. We conducted to investigate the relationships between acute exercise and CXCR4 expression on natural killer (NK) cells. Peripheral blood mononuclear cells (PBMCs) were cultured with cortisol and analyzed for CXCR4 expression CD3(-)/CD56(+) NK cells and their migration activity. To determine the effect of exercise, we isolated PBMCs from subjects before and after a 90-min exercise at 70% peak O2 uptake (VO2 peak) and determined the changes in CXCR4 expression on their NK cells after exercise. We cultured PBMCs with plasma obtained before and after exercise and/or the glucocorticoid antagonist RU-486 to determine NK cell migration activity and the effects of cortisol on CXCR4 expression in vitro. Cortisol treatment increased CXCR4 expression on NK cells (P < 0.05) and their migration activity (P < 0.05). Exercise did not affect CXCR4 expression on NK cells, whereas incubating NK cells with post-exercise plasma significantly increased CXCR4 expression (P < 0.05) and their migration activity (P < 0.05). RU-486 blocked cortisol-induced CXCR4 upregulation on NK cells, but only partially blocked (7%) CXCR4 upregulation when PMBCs were incubated with post-exercise plasma. Thus, acute exercise increases CXCR4 expression on NK cells and their migration activity and may contribute to NK cell redistribution after acute exercise; however, cortisol did not appear to be the primary mediator of augmented CXCR4 expression.
Mitsuharu Okutsu; Kenji Ishii; Kaijun Niu; Ryoichi Nagatomi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-6-19
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  -     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2014 Jun 
Date Detail:
Created Date:  2014-6-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014, Journal of Applied Physiology.
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