| Cortisol differentially alters claudin isoforms in cultured puffer fish gill epithelia. | |
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MedLine Citation:
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PMID: 19969041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A primary cultured gill epithelium from the puffer fish Tetraodon nigroviridis was developed to examine the corticosteroid regulation of claudin isoform mRNA abundance in fish gills. Preparations were composed of polygonal epithelial cells exhibiting concentric apical microridges and zonula occludens-1 immunoreactivity along cell margins. No evidence was found to indicate the presence of Na(+)-K(+)-ATPase-immunoreactive or mitochondria-rich cells in cultured preparations. Therefore, epithelia appear to be composed of gill pavement cells (PVCs) only. An RT-PCR profile of 12 salinity responsive gill claudin tight junction (TJ) proteins (Tncldn3a, -3c, -6, -8d, -10d, -10e, -11a, -23b, -27a, -27c, -32a, and -33b) revealed the absence of Tncldn6, -10d and -10e in cultured epithelia, suggesting that these isoforms are not associated with gill PVCs. Cortisol treatment of cultured epithelia dose-dependently increased or decreased mRNA abundance of select claudin isoforms. Transcript abundance of several claudin isoforms was unaffected by cortisol treatment. These data provide evidence for the cell specific distribution of claudins in fish gills and suggest that heterogeneous alterations in the abundance of select claudin isoforms contribute to the corticosteroid regulation of gill permeability. |
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Authors:
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Phuong Bui; Mazdak Bagherie-Lachidan; Scott P Kelly |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-05 |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 317 ISSN: 1872-8057 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
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Languages: eng Pagination: 120-6 Citation Subset: IM |
Affiliation:
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Department of Biology, York University, 4700 Keele Street, Toronto, ON, Canada M3J 1P3. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acclimatization
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drug effects Animals Claudins / genetics, metabolism* Epithelium / drug effects*, metabolism* Fresh Water Gene Expression Profiling Gene Expression Regulation / drug effects Gills / drug effects, metabolism* Hydrocortisone / pharmacology* Immunohistochemistry Protein Isoforms / genetics, metabolism RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Salinity Seawater Tetraodontiformes / genetics, metabolism* Tissue Culture Techniques |
| Chemical | |
Reg. No./Substance:
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0/Claudins; 0/Protein Isoforms; 0/RNA, Messenger; 50-23-7/Hydrocortisone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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