Document Detail

Cortisol assays and diagnostic laboratory procedures in human biological fluids.
MedLine Citation:
PMID:  19414006     Owner:  NLM     Status:  MEDLINE    
The overview of cortisol physiology, action and pathology is achieved in relation to the hypothalamic-pituitary-adrenal axis alteration by laboratory investigation. The measurements of cortisol and related compound levels in blood, urine and saliva used to study the physiological and pathological cortisol involvement, are critically reviewed. The immunoassay and chromatographic methods for cortisol measurement in the various biological fluids are examined in relation to their analytical performances, reference ranges and diagnostic specificity and sensitivity. Moreover, blood, urine and saliva cortisol level measurements are described taking into account the diagnostic implications. The deduction is that each method requires the definition of its own reference range and its related diagnostic cut-off levels. Thus, this review, stressing the analysis procedures, could help to understand and compare the results of the different assays.
Rosalba Gatti; Giorgia Antonelli; Maddalena Prearo; Paolo Spinella; Enrico Cappellin; Elio F De Palo
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Publication Detail:
Type:  Journal Article; Review     Date:  2009-05-04
Journal Detail:
Title:  Clinical biochemistry     Volume:  42     ISSN:  1873-2933     ISO Abbreviation:  Clin. Biochem.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-27     Completed Date:  2009-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1205-17     Citation Subset:  IM    
Department of Medical Diagnostic and Spec. Ther., University of Padova (I), Italy.
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MeSH Terms
Addison Disease / diagnosis,  metabolism,  physiopathology
Biological Assay / instrumentation,  methods*
Body Fluids / chemistry*
Circadian Rhythm / physiology
Cushing Syndrome / diagnosis,  metabolism,  physiopathology
Hydrocortisone* / chemistry,  metabolism
Laboratory Techniques and Procedures*
Pituitary-Adrenal System / physiology
Reference Values
Reproducibility of Results
Saliva / chemistry
Sensitivity and Specificity
Signal Transduction / physiology
Specimen Handling
Stress, Physiological
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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