Document Detail

Corticotropin-releasing hormone receptor subtype 1 is significantly up-regulated at the time of labor in the human myometrium.
MedLine Citation:
PMID:  9814500     Owner:  NLM     Status:  MEDLINE    
Circulating concentrations of CRH rise late in human pregnancy, reaching a peak at labor. The presence of functional CRH receptors, CRH-R1 and CRH-R2, in the human myometrium suggests that CRH may modulate uterine activity. We hypothesized that the number of CRH receptors would be higher in myometrium than fetal membranes (FM) and would change during labor. Myometrial samples were collected from the lower segment (LS) in nonpregnant, preterm (32 +/- 2 weeks), and term (39 +/- 1.6 weeks) pregnant patients before and at labor. Fundus and LS samples were also collected from nonpregnant, pregnant, laboring, and postpartum women. FM were collected at term and at labor. We identified CRH receptors in myometrium and FM by semiquantitative RT-PCR and immunohistochemistry. CRH-R1 messenger ribonucleic acid (mRNA) in the LS was decreased in pregnancy and increased significantly in both preterm and term labor (P < 0.05), but remained unchanged in the fundus. CRH-R2 mRNA was present in 28% of LS myometrium with no change at labor. CRH-R1 and CRH-R2 protein was localized to myometrial smooth muscle in nonpregnant and laboring patients, with lower levels at term. CRH-R1 mRNA was present in chorion and decidua, but CRH-R2 was undetectable in these tissues. We conclude that CRH-R1 is expressed preferentially in myometrium and FM. Changes in CRH receptors during labor are consistent with CRH mediating effects on myometrial activity.
M Y Stevens; J R Challis; S J Lye
Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  83     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-11-25     Completed Date:  1998-11-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4107-15     Citation Subset:  AIM; IM    
Medical Research Council Group in Fetal and Neonatal Health and Development, Department of Physiology, University of Toronto, Mount Sinai Hospital, Ontario, Canada.
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MeSH Terms
Decidua / metabolism
Extraembryonic Membranes / metabolism
Labor Onset
Labor, Obstetric / metabolism*
Myometrium / metabolism*
Postpartum Period
Pregnancy Proteins / biosynthesis
Receptors, Corticotropin-Releasing Hormone / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Pregnancy Proteins; 0/Receptors, Corticotropin-Releasing Hormone

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