Document Detail

Corticotropin-releasing hormone modulates human trophoblast invasion through carcinoembryonic antigen-related cell adhesion molecule-1 regulation.
MedLine Citation:
PMID:  16400017     Owner:  NLM     Status:  MEDLINE    
Abnormalities in the process of trophoblast invasion may result in abnormal placentation. Both the embryonic trophoblast and maternal decidua produce corticotropin-releasing hormone (CRH), which promotes implantation. Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), which is expressed in extravillous trophoblasts (EVTs) of normal human placenta, may also function in tro-phoblast/endometrial interactions. We investigated whether locally produced CRH plays a role in trophoblast invasion, primarily by regulating CEACAM1 expression. We examined cultures of freshly isolated human EVTs, which express CEACAM1, and an EVT-based hybridoma cell line, which is devoid of endogenous CEACAM1. CRH inhibited EVT invasion in Matrigel invasion assays, and this effect was blocked by the CRH receptor type 1 (CRHR1)-specific antagonist antalarmin. Additionally, CRH decreased CEACAM1 expression in EVTs in a dose-dependent manner. After transfection of the hybridoma cell line with a CEACAM1 expression vector, the invasiveness of these cells was strongly enhanced. This effect was inhibited by addition of blocking monoclonal antibody against CEACAM1. Furthermore, blocking of endogenous CEACAM1 in EVTs inhibited the invasive potential of these cells. Taken together these findings suggest that CRH inhibits trophoblast invasion by decreasing the expression of CEACAM1 through CRHR1, an effect that might be involved in the pathophysiology of clinical conditions, such as preeclampsia and placenta accreta.
Ana-Maria Bamberger; Vassilis Minas; Sophia N Kalantaridou; Jessica Radde; Helen Sadeghian; Thomas Löning; Ioannis Charalampopoulos; Jens Brümmer; Christoph Wagener; Christoph M Bamberger; Heinrich M Schulte; George P Chrousos; Antonis Makrigiannakis
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  The American journal of pathology     Volume:  168     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-09     Completed Date:  2006-03-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  141-50     Citation Subset:  AIM; IM    
Department of Gynecopathology, Institute of Pathology, University Hospital Eppendorf, Hamburg, Germany.
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MeSH Terms
Antigens, CD / metabolism*
Blotting, Western
Cell Adhesion Molecules / metabolism*
Cell Line
Cell Movement / physiology
Corticotropin-Releasing Hormone / metabolism*
Flow Cytometry
Trophoblasts / metabolism*
Reg. No./Substance:
0/Antigens, CD; 0/CD66 antigens; 0/Cell Adhesion Molecules; 9015-71-8/Corticotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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